Detailed information of TA system TA204689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2325577..2326154
• Replicon: chromosome
• Accession: NZ_CP076134
• Organism: Bradyrhizobium sediminis strain S2-20-1

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: KMZ29_RS11100
• Protein ID: WP_215623717.1
• Coordinates: 2325873..2326154 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KMZ29_RS11095
• Protein ID: WP_215623716.1
• Coordinates: 2325577..2325861 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KMZ29_RS11075 (KMZ29_11075)	2320655..2321788	+	1134	WP_215623712.1	glycosyltransferase family 2 protein	-
KMZ29_RS11080 (KMZ29_11080)	2321796..2323298	+	1503	WP_215623713.1	glycosyltransferase family 39 protein	-
KMZ29_RS11085 (KMZ29_11085)	2323501..2324238	+	738	WP_215623714.1	DUF899 family protein	-
KMZ29_RS11090 (KMZ29_11090)	2324448..2325566	+	1119	WP_215623715.1	DUF2778 domain-containing protein	-
KMZ29_RS11095 (KMZ29_11095)	2325577..2325861	-	285	WP_215623716.1	HigA family addiction module antitoxin	Antitoxin
KMZ29_RS11100 (KMZ29_11100)	2325873..2326154	-	282	WP_215623717.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KMZ29_RS11105 (KMZ29_11105)	2326259..2326855	-	597	WP_215623718.1	cysteine hydrolase family protein	-
KMZ29_RS11110 (KMZ29_11110)	2326985..2327827	+	843	WP_215623719.1	LysR family transcriptional regulator	-
KMZ29_RS11115 (KMZ29_11115)	2327993..2329636	-	1644	WP_215623720.1	chaperonin GroEL	-
KMZ29_RS11120 (KMZ29_11120)	2329778..2330095	-	318	WP_215606075.1	co-chaperone GroES	-
KMZ29_RS11125 (KMZ29_11125)	2330475..2330762	+	288	WP_215623721.1	usg protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-83)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10714.32 Da
• Isoelectric Point: 9.3512

>T204689 WP_215623717.1 NZ_CP076134:c2326154-2325873 [Bradyrhizobium sediminis]
MIRSFADKATELLFRGGPCPARWRSIEAVALRKLDMVDAATRLDDLLSPPGNRLEALKGDRRGQHSIRINRQWRICFRWT
PDGPEAIEIVDYH

Nucleotide

• Length: 282 bp

>T204689 NZ_CP101210:4552060-4552470 [Escherichia coli]
ATGAAATTATTACCCGACACACATGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGTCTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCCGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATCGATATTCTCCGGGCCCGCCGGGCAACAGGCCTGATGACACGTAA
TAACTACAGAATGGTAAATAACATTACCCTGGGTAGCATCCGGGGGCGAAACGATGAAACTTTCCCTGACGCTGGAAGCC
GACAGCATTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10691.37 Da
• Isoelectric Point: 10.3620

>AT204689 WP_215623716.1 NZ_CP076134:c2325861-2325577 [Bradyrhizobium sediminis]
MARLRTHPGEVLKEEYLVPLGLSARVLAKELDIPANRLTEIMRGARDVSADTAIRLGRYFRTDPRFWLNLQAAYDLSKAE
NTHSYKKIVPRPAA

Nucleotide

• Length: 285 bp

>AT204689 NZ_CP101210:4551601-4551969 [Escherichia coli]
GTGTCAGACACACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGACATCAGAGGCCGGT
TCAGCGATGCGGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA