Detailed information of TA system TA204678

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4327399..4328001
• Replicon: chromosome
• Accession: NZ_CP076123
• Organism: Escherichia coli strain F11

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: F11EC_RS20695
• Protein ID: WP_000897305.1
• Coordinates: 4327399..4327710 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F11EC_RS20700
• Protein ID: WP_000356397.1
• Coordinates: 4327711..4328001 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F11EC_RS20670 (4323313)	4323313..4323912	+	600	WP_001298594.1	glucose-1-phosphatase	-
F11EC_RS20675 (4323906)	4323906..4324778	+	873	WP_000920762.1	virulence factor BrkB family protein	-
F11EC_RS20680 (4324775)	4324775..4325212	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
F11EC_RS20685 (4325257)	4325257..4326198	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
F11EC_RS20690 (4326262)	4326262..4327170	-	909	WP_001298596.1	alpha/beta hydrolase	-
F11EC_RS20695 (4327399)	4327399..4327710	+	312	WP_000897305.1	hypothetical protein	Toxin
F11EC_RS20700 (4327711)	4327711..4328001	+	291	WP_000356397.1	NadS family protein	Antitoxin
F11EC_RS20705 (4328606)	4328606..4328824	+	219	WP_001298592.1	CopG family transcriptional regulator	-
F11EC_RS20710 (4329039)	4329039..4329968	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
F11EC_RS20715 (4329965)	4329965..4330600	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
F11EC_RS20720 (4330597)	4330597..4331499	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12172.16 Da
• Isoelectric Point: 9.7791

>T204678 WP_000897305.1 NZ_CP076123:4327399-4327710 [Escherichia coli]
ILFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T204678 NZ_CP101210:2264546-2264648 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT204678 WP_000356397.1 NZ_CP076123:4327711-4328001 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT204678 NZ_CP101210:c2264686-2264418 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7