Detailed information of TA system TA204675

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3502352..3503151
• Replicon: chromosome
• Accession: NZ_CP076123
• Organism: Escherichia coli strain F11

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: F11EC_RS16700
• Protein ID: WP_000347252.1
• Coordinates: 3502687..3503151 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: F11EC_RS16695
• Protein ID: WP_001296435.1
• Coordinates: 3502352..3502687 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F11EC_RS16680 (3498137)	3498137..3498907	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
F11EC_RS16685 (3498923)	3498923..3500257	-	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
F11EC_RS16690 (3500632)	3500632..3502203	+	1572	WP_001273939.1	galactarate dehydratase	-
F11EC_RS16695 (3502352)	3502352..3502687	+	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
F11EC_RS16700 (3502687)	3502687..3503151	+	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
F11EC_RS16705 (3503206)	3503206..3504015	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
F11EC_RS16710 (3504264)	3504264..3505544	+	1281	WP_000681930.1	tagatose-bisphosphate aldolase subunit KbaZ	-
F11EC_RS16715 (3505567)	3505567..3506040	+	474	WP_001298322.1	PTS N-acetylgalactosamine transporter subunit IIB	-
F11EC_RS16720 (3506051)	3506051..3506830	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
F11EC_RS16725 (3506820)	3506820..3507698	+	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
F11EC_RS16730 (3507716)	3507716..3508150	+	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T204675 WP_000347252.1 NZ_CP076123:3502687-3503151 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T204675 NZ_CP101210:1492494-1492892 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAACATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT204675 WP_001296435.1 NZ_CP076123:3502352-3502687 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT204675 NZ_CP101210:1492267-1492494 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5