Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 2670382..2671061 Replicon chromosome
Accession NZ_CP076123
Organism Escherichia coli strain F11

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag F11EC_RS12855 Protein ID WP_000057523.1
Coordinates 2670759..2671061 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag F11EC_RS12850 Protein ID WP_000806442.1
Coordinates 2670382..2670723 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F11EC_RS12840 (2666626) 2666626..2667558 - 933 WP_000883025.1 glutaminase A -
F11EC_RS12845 (2667820) 2667820..2670324 + 2505 WP_000083943.1 copper-exporting P-type ATPase CopA -
F11EC_RS12850 (2670382) 2670382..2670723 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
F11EC_RS12855 (2670759) 2670759..2671061 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
F11EC_RS12860 (2671194) 2671194..2671988 + 795 WP_000365148.1 TraB/GumN family protein -
F11EC_RS12865 (2672192) 2672192..2672671 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
F11EC_RS12870 (2672695) 2672695..2673495 + 801 WP_000439798.1 hypothetical protein -
F11EC_RS12875 (2673492) 2673492..2673995 + 504 WP_000667000.1 hypothetical protein -
F11EC_RS12880 (2674033) 2674033..2675685 - 1653 WP_000771760.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T204669 WP_000057523.1 NZ_CP076123:c2671061-2670759 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T204669 NZ_CP101210:273833-273940 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT204669 WP_000806442.1 NZ_CP076123:c2670723-2670382 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT204669 NZ_CP101210:c273776-273719 [Escherichia coli]
TCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References