Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1717014..1717576 Replicon chromosome
Accession NZ_CP076123
Organism Escherichia coli strain F11

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag F11EC_RS08130 Protein ID WP_000605675.1
Coordinates 1717014..1717292 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag F11EC_RS08135 Protein ID WP_000781370.1
Coordinates 1717292..1717576 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F11EC_RS08105 (1712600) 1712600..1713031 - 432 WP_000152305.1 peroxiredoxin OsmC -
F11EC_RS08110 (1713376) 1713376..1713591 + 216 WP_000495766.1 biofilm-dependent modulation protein -
F11EC_RS08115 (1713693) 1713693..1713830 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
F11EC_RS08120 (1713987) 1713987..1715684 + 1698 WP_000433462.1 malate dehydrogenase -
F11EC_RS08125 (1715818) 1715818..1716828 + 1011 WP_000642380.1 alcohol dehydrogenase AdhP -
F11EC_RS08130 (1717014) 1717014..1717292 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
F11EC_RS08135 (1717292) 1717292..1717576 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
F11EC_RS08140 (1717805) 1717805..1718458 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
F11EC_RS08145 (1718451) 1718451..1719335 - 885 WP_001240581.1 formate dehydrogenase N subunit beta -
F11EC_RS08150 (1719348) 1719348..1722395 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T204668 WP_000605675.1 NZ_CP076123:1717014-1717292 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T204668 NZ_CP101210:273350-273457 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT204668 WP_000781370.1 NZ_CP076123:1717292-1717576 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT204668 NZ_CP101210:c273302-273236 [Escherichia coli]
GGTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References