Detailed information of TA system TA204656

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 284855..285549
• Replicon: chromosome
• Accession: NZ_CP076123
• Organism: Escherichia coli strain F11

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: F11EC_RS01310
• Protein ID: WP_001263500.1
• Coordinates: 285151..285549 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: F11EC_RS01305
• Protein ID: WP_000554758.1
• Coordinates: 284855..285148 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F11EC_RS01290 (281208)	281208..282920	-	1713	Protein_253	flagellar biosynthesis protein FlhA	-
F11EC_RS01295 (282892)	282892..283677	+	786	WP_000207578.1	putative lateral flagellar export/assembly protein LafU	-
F11EC_RS01300 (283748)	283748..284803	+	1056	WP_001226168.1	DNA polymerase IV	-
F11EC_RS01305 (284855)	284855..285148	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
F11EC_RS01310 (285151)	285151..285549	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
F11EC_RS01315 (285559)	285559..286011	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
F11EC_RS01320 (286201)	286201..287340	+	1140	WP_000521561.1	RNA ligase RtcB family protein	-
F11EC_RS01325 (287337)	287337..287951	+	615	WP_000602124.1	peptide chain release factor H	-
F11EC_RS01330 (288008)	288008..289465	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
F11EC_RS01335 (289726)	289726..290184	+	459	WP_001291991.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	263894..286011	22117

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T204656 WP_001263500.1 NZ_CP076123:285151-285549 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T204656 NZ_CP101197:c4425373-4425023 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGTGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT204656 WP_000554758.1 NZ_CP076123:284855-285148 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT204656 NZ_CP101197:c4425618-4425367 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3