Detailed information of TA system TA204644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 1521232..1521884
• Replicon: plasmid unnamed1
• Accession: NZ_CP076116
• Organism: Ralstonia solanacearum strain Rs-SY1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0S4U5H4
• Locus tag: KME70_RS23140
• Protein ID: WP_028861315.1
• Coordinates: 1521232..1521576 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KME70_RS23145
• Protein ID: WP_020425351.1
• Coordinates: 1521573..1521884 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KME70_RS23125 (KME70_23125)	1516928..1518091	-	1164	WP_016726526.1	2-methylcitrate synthase	-
KME70_RS23130 (KME70_23130)	1518143..1519039	-	897	WP_071092368.1	methylisocitrate lyase	-
KME70_RS23135 (KME70_23135)	1519232..1521181	+	1950	WP_023470006.1	propionate catabolism operon regulatory protein PrpR	-
KME70_RS23140 (KME70_23140)	1521232..1521576	+	345	WP_028861315.1	hypothetical protein	Toxin
KME70_RS23145 (KME70_23145)	1521573..1521884	+	312	WP_020425351.1	transcriptional regulator	Antitoxin
KME70_RS23150 (KME70_23150)	1522024..1522833	-	810	WP_071623829.1	DUF2242 domain-containing protein	-
KME70_RS23155 (KME70_23155)	1523477..1524136	-	660	WP_149079161.1	hypothetical protein	-
KME70_RS23160 (KME70_23160)	1524507..1526312	+	1806	WP_237177646.1	3'-5' exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG / acrB / cheY / cheB / cheW / motA / cyaB / flgC / flgG / fliM / fliG / fliI	1..2055017	2055017

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13276.03 Da
• Isoelectric Point: 9.3395

>T204644 WP_028861315.1 NZ_CP076116:1521232-1521576 [Ralstonia solanacearum]
MDATFIELPPFQRLREHYLSDDEYRALQQELLARPDAGDVIKGTGGLRKLRFSDKRRGKGKRGGLRVIYYHWDGGGQFWM
FVVYDKGEADDLSSDERKVLARLLEQEVKARSER

Nucleotide

• Length: 345 bp

>T204644 NZ_CP101197:1197763-1198074 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11619.65 Da
• Isoelectric Point: 10.7132

>AT204644 WP_020425351.1 NZ_CP076116:1521573-1521884 [Ralstonia solanacearum]
MKRNLFAELKEGMDALAAEREGKMTLRKVKVRVLQPLDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT204644 NZ_CP101197:1198071-1198490 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCCTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0S4U5H4