Detailed information of TA system TA204617

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 118159..118790
• Replicon: plasmid p352-1
• Accession: NZ_CP076061
• Organism: Cupriavidus sp. EM10

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: KLP38_RS17650
• Protein ID: WP_215531251.1
• Coordinates: 118159..118437 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KLP38_RS17655
• Protein ID: WP_215531252.1
• Coordinates: 118476..118790 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KLP38_RS17640 (KLP38_17640)	117437..117646	+	210	WP_255640178.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KLP38_RS17645 (KLP38_17645)	117643..118023	+	381	WP_215531250.1	helix-turn-helix domain-containing protein	-
KLP38_RS17650 (KLP38_17650)	118159..118437	+	279	WP_215531251.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KLP38_RS17655 (KLP38_17655)	118476..118790	+	315	WP_215531252.1	HigA family addiction module antitoxin	Antitoxin
KLP38_RS17660 (KLP38_17660)	118816..119322	-	507	WP_215531253.1	peptide-methionine (S)-S-oxide reductase MsrA	-
KLP38_RS17665 (KLP38_17665)	119391..120005	+	615	WP_215531979.1	uracil-DNA glycosylase family protein	-
KLP38_RS17670 (KLP38_17670)	120017..121243	-	1227	WP_225934624.1	cation:proton antiporter	-
KLP38_RS17675 (KLP38_17675)	121347..121817	-	471	WP_066739987.1	Lrp/AsnC family transcriptional regulator	-
KLP38_RS17680 (KLP38_17680)	121963..122889	+	927	WP_215531254.1	DMT family transporter	-
KLP38_RS17685 (KLP38_17685)	122899..123369	-	471	WP_215531255.1	Lrp/AsnC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	plc / acrB / hcp/tssD / vipB/tssC / katA / gmd / motA / cheW / cheB / cheY / cheZ / flhA / flgC / flgG / fliI / ddhA / katA / adeG / katA	1..2510418	2510418

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (30-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-94)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10428.91 Da
• Isoelectric Point: 6.9463

>T204617 WP_215531251.1 NZ_CP076061:118159-118437 [Cupriavidus sp. EM10]
MIVSSSCRLTPALFAGQRVPRFVNFGTTAERKLAMLEAAVILEDLATPPGNRLERLYSDRPDQYSIRINDQWRICFTWTS
DGPANVAIIDYH

Nucleotide

• Length: 279 bp

>T204617 NZ_CP101169:3783271-3783762 [Xanthomonas campestris pv. campestris]
ATGACGGTATCGGCGCCGGAACCGCTGACAGCGCATCACCGGGTCGAACCGTTCGCATCGGGCGTGGAGAGCCTGGATCA
CTGGCTCAAGCGCCGCGCCCTGAAAAATCAGGCAACCGGTGCGTCACGGACCTTCGTTGCCTGCGAGGATGACCGCGTGG
TGGCCTATTACGCCCTGGCGTCGAGTGCGGTCGCCGTGGACGCGACGCCCGGCCGCTTCCGTCGCAACATGCCGGACCCG
ATCCCCGTGGTGGTGCTTGGGAGGCTTGCTGTTGACCAATCCCTGCATGGCAGAGGTTTTGGCCGCGCCCTGATGCAGGA
TGCTGGCAAGCGCATCCTGCATGCGGCCGACACCATCGGCATTCGCGGGCTGCTCGTGCATGCGCTCTCGGCGGACGCCA
AGGCGTTTTACGAGCGCATCGGCTTCGAACCTTCCCCGCTGGATCCCATGGTGCTGGTCGTCACGCTGGAAGACCTGAAA
GCCAGTCTGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11763.58 Da
• Isoelectric Point: 6.2263

>AT204617 WP_215531252.1 NZ_CP076061:118476-118790 [Cupriavidus sp. EM10]
MQKIENGLRPVHPGEVLREEFLVPLDMTANALAGMLHVTPARINEIVRGERGVTPVTALRLARYFGGTAEFWMNLQQTYD
LKIARLEHEAEIFAEIQPRQVNPA

Nucleotide

• Length: 315 bp

>AT204617 NZ_CP101169:3782984-3783274 [Xanthomonas campestris pv. campestris]
ATGTCCACACCCAACACTACGCCTGGCAAGCGCGAAACCTTGAATCTCCGCATCAAGCCGGAGGAGCGCAGCCTGATCGA
CCGGGCCGCCAAGGCGCGCGGGAAGAACCGCACCGATTTCATGCTGGACGCAGCGCGCTCGGCGGCCGAGGAAGCCTTGC
TGGACCAGACCCTCATCACGGCCAGCCCGGACGCCTACGCCGCCTTTTTGGCACGGCTGGACATGCCGCCGCAGCCGAAC
GCGCGCCTGCGCAAGACGATGCAGACGCCCGCTCCTTGGGAGAAGGCATGA