Detailed information of TA system TA204614

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1481896..1482537
• Replicon: chromosome
• Accession: NZ_CP076050
• Organism: Cupriavidus metallidurans strain YL2

Toxin (Protein)

• Gene name: graT
• Uniprot ID: L2E9B9
• Locus tag: KB891_RS23315
• Protein ID: WP_008651239.1
• Coordinates: 1481896..1482180 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KB891_RS23320
• Protein ID: WP_017514877.1
• Coordinates: 1482190..1482537 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KB891_RS23290 (KB891_23290)	1477393..1477983	-	591	WP_215242617.1	hypothetical protein	-
KB891_RS23295 (KB891_23295)	1478108..1478794	-	687	WP_215242618.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
KB891_RS23300 (KB891_23300)	1479147..1480037	-	891	WP_024569403.1	helix-turn-helix transcriptional regulator	-
KB891_RS23305 (KB891_23305)	1480348..1480566	+	219	WP_008651241.1	YdcH family protein	-
KB891_RS23310 (KB891_23310)	1480568..1481719	+	1152	WP_215242619.1	polyphosphate kinase 2	-
KB891_RS23315 (KB891_23315)	1481896..1482180	+	285	WP_008651239.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KB891_RS23320 (KB891_23320)	1482190..1482537	+	348	WP_017514877.1	HigA family addiction module antitoxin	Antitoxin
KB891_RS23325 (KB891_23325)	1482652..1483965	+	1314	WP_215242620.1	hemolysin family protein	-
KB891_RS23330 (KB891_23330)	1484365..1485702	+	1338	WP_215242621.1	EAL domain-containing protein	-
KB891_RS23335 (KB891_23335)	1485862..1485975	+	114	WP_223277463.1	entericidin A/B family lipoprotein	-
KB891_RS23340 (KB891_23340)	1486094..1486735	-	642	WP_215242622.1	bifunctional 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (7-84)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-85)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10505.11 Da
• Isoelectric Point: 7.9133

>T204614 WP_008651239.1 NZ_CP076050:1481896-1482180 [Cupriavidus metallidurans]
MIRSLSDKTTAAIYCGQSVHTLPAHIQADALRKLAILDAASSLPDMVAPPANRLEPVLGKRRGHWRVRIQGPWHLCFRFI
NGEAWDVGIAEYEE

Nucleotide

• Length: 285 bp

>T204614 NZ_CP101140:c4791989-4791738 [Streptomyces cavourensis]
ATGCGCACGATGACCTACACCGAGTCGCGGGCGAAGTACGCGGAAACCCTGAGCGCGGTCGTGAACGACCGCGAGGAGGT
CGTGGTCACGCGGGCGGGCCATGAGCCCGTGGTGATAGTGGCGCTCGACGAGTACGAGTCGCTCAAGGAGACCGCGTACC
AGCTCCGCAGCCCGGAGAACGCCCGTCGGCTGCTGGCCTCCATCGAGCGCCTGGAGAGCGGGGGCGGCACGGTGCGGGAG
CTGGCGGAGTGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 12712.65 Da
• Isoelectric Point: 9.9584

>AT204614 WP_017514877.1 NZ_CP076050:1482190-1482537 [Cupriavidus metallidurans]
MTIKREDLRRADFRSIATGRPIPPAHPGEVLETEFMAPNELSAEALAVALHMAPQRIHDIVRRQRAISPDTALRLARYFG
TSAEFWAGLQAAYALRLARAAAGPIIERDIQPLTA

Nucleotide

• Length: 348 bp

>AT204614 NZ_CP101140:c4791741-4791481 [Streptomyces cavourensis]
GTGAAGTTCGTATGGGATCAGTCGTCCTGGGACGACTACGTCTGGTGGCAGAACCAGGACCGCAAGATCCTCAAGCGGAT
CAACACGCTGCTCCAGGACATCGCCCGGAACGGCAACGAGGGCATGGGCAAGCCGGAGCCCCTGAAGCACGGATTCCAGG
GGTACTGGTCGCGCCGCATCACCGATGAGCACCGGCTCATCTACAAGGTGGTCGACGACGAGGTGCGTGTCGCCGCCTGC
CGCTACCACTACGGGCGCTGA

Structures

Toxin

• AlphaFold DB: L2E9B9