Detailed information of TA system TA204600

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 4116941..4117634
• Replicon: chromosome
• Accession: NZ_CP076043
• Organism: Escherichia coli strain Stbl4

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: ITN70_RS19975
• Protein ID: WP_000415584.1
• Coordinates: 4116941..4117237 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: ITN70_RS19980
• Protein ID: WP_000650107.1
• Coordinates: 4117239..4117634 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITN70_RS19940 (4112029)	4112029..4112343	-	315	WP_000958598.1	putative quinol monooxygenase	-
ITN70_RS19945 (4112374)	4112374..4112955	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
ITN70_RS19950 (4113274)	4113274..4113606	+	333	WP_000917684.1	DUF2645 family protein	-
ITN70_RS19955 (4113652)	4113652..4115001	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
ITN70_RS19960 (4114998)	4114998..4115657	-	660	WP_001221495.1	quorum sensing response regulator transcription factor QseB	-
ITN70_RS19965 (4115809)	4115809..4116201	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
ITN70_RS19970 (4116254)	4116254..4116736	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
ITN70_RS19975 (4116941)	4116941..4117237	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
ITN70_RS19980 (4117239)	4117239..4117634	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
ITN70_RS19985 (4117767)	4117767..4119374	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
ITN70_RS19990 (4119512)	4119512..4121770	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T204600 WP_000415584.1 NZ_CP076043:4116941-4117237 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T204600 NZ_CP101125:c3050166-3049885 [Pseudomonas nunensis]
ATGACCTATGAGCTGGAGTTTTCTGAAAAGGCCTGGAAAGAATGGAAGAAGCTTGGGGTAAACCTTAGGGAGCAGTTCAA
AAATAAGCTACAGGAGCGCCTTGTCCACCCGCACGTGCCAGCAGATCGACTCCATGGGCTTGGGAATGCTTACAAAATCA
AGCTGCGGAGCGCGGGGTATCGATTGGTTTATCGGGTGAAAGACGAGGTGCTGATAGTGACCGTAATTGCAGTGGGTAAA
AGAGAACGTGGCGGTGTTTACAAAGATGCAGGCGAGCGATAG

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT204600 WP_000650107.1 NZ_CP076043:4117239-4117634 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT204600 NZ_CP101125:c3050404-3050156 [Pseudomonas nunensis]
ATGACACATATCGTGCTTTCGCAAGTTGTCGCGAGCATATCTGAACTAAAAAAAAACCCGATGGGCACAGTGGCGGCCGG
CGGTGGGCTATCTGTTGCGATTCTCAACCGCAACGAGCCGGCGTTCTATTGTGTTCCAGCCAAAGAGTACGAGGCCATGA
TGAGTCGTCTGGAAGATTTGGAATTGATCGCGCTTTGTAAAGAAAGAGAAAACGACCCAACCGTAAAGGTTTCAATTGAT
GACCTATGA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65