204599

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4051031..4051758 Replicon chromosome
Accession NZ_CP076043
Organism Escherichia coli strain Stbl4

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag ITN70_RS19665 Protein ID WP_000550189.1
Coordinates 4051031..4051345 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag ITN70_RS19670 Protein ID WP_000560266.1
Coordinates 4051342..4051758 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ITN70_RS19645 (4047189) 4047189..4048175 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
ITN70_RS19650 (4048254) 4048254..4048946 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
ITN70_RS19655 (4049023) 4049023..4049526 - 504 WP_001333820.1 M48 family metallopeptidase -
ITN70_RS19660 (4049611) 4049611..4050747 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
ITN70_RS19665 (4051031) 4051031..4051345 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
ITN70_RS19670 (4051342) 4051342..4051758 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
ITN70_RS19675 (4051803) 4051803..4053821 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
ITN70_RS19680 (4054247) 4054247..4056598 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T204599 WP_000550189.1 NZ_CP076043:4051031-4051345 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T204599 NZ_CP101125:c179438-179184 [Pseudomonas nunensis]
ATGCAAACCATCAACTACACCACTGCGCGTGCACACTTGGCTGAAACCATGGATCGGGTCAATGAAGATCGTGCGCCCTT
GCTGGTAACTCGCCAGAAGGGCGAACCGGTGGTGATGATGTCACTAGCAGAGTACAACGCGCTCGAAGAGACCGCATATT
TGCTCCGATCCCCAGCCAATGCTGAACGCTTGATCAAATCAATTGGCAACCTGCGAGCAGGGAAAACCAAGGCCAGGCGC
CTTATCGAAGAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT204599 WP_000560266.1 NZ_CP076043:4051342-4051758 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT204599 NZ_CP101125:c179187-178933 [Pseudomonas nunensis]
ATGAATATCGAGTTCACTCCTGAAGGTTGGGACGACTACTTATGGTTCCAGCAAAACGATAAAGCCGGGCTCAAACGAAT
CAACCTTTTGATCAAAGCGATCCAGCGTGAGCCTTTCAGCGGCCCCGGAAAACCTGAGCCGCTCAAATACAATCTGAGCG
GCTTCTGGTCTCGCCGGATTACAGCGGAGCATCGCTTGGTCTATGCGATCGAAGATGGCGCGATACAGATCGTTATGTGC
AGATACCATTACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References