Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4005011..4005810 Replicon chromosome
Accession NZ_CP076043
Organism Escherichia coli strain Stbl4

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag ITN70_RS19425 Protein ID WP_000347273.1
Coordinates 4005011..4005475 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag ITN70_RS19430 Protein ID WP_001307405.1
Coordinates 4005475..4005810 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ITN70_RS19400 (4001187) 4001187..4001744 - 558 Protein_3793 amidohydrolase family protein -
ITN70_RS19405 (4001740) 4001740..4002111 - 372 Protein_3794 PTS sugar transporter subunit IIC -
ITN70_RS19410 (4002122) 4002122..4002595 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
ITN70_RS19415 (4002618) 4002618..4003898 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
ITN70_RS19420 (4004147) 4004147..4004956 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
ITN70_RS19425 (4005011) 4005011..4005475 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
ITN70_RS19430 (4005475) 4005475..4005810 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
ITN70_RS19435 (4005959) 4005959..4007530 - 1572 WP_001273747.1 galactarate dehydratase -
ITN70_RS19440 (4007905) 4007905..4009239 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
ITN70_RS19445 (4009255) 4009255..4010025 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T204598 WP_000347273.1 NZ_CP076043:c4005475-4005011 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T204598 NZ_CP101125:c179187-178933 [Pseudomonas nunensis]
ATGAATATCGAGTTCACTCCTGAAGGTTGGGACGACTACTTATGGTTCCAGCAAAACGATAAAGCCGGGCTCAAACGAAT
CAACCTTTTGATCAAAGCGATCCAGCGTGAGCCTTTCAGCGGCCCCGGAAAACCTGAGCCGCTCAAATACAATCTGAGCG
GCTTCTGGTCTCGCCGGATTACAGCGGAGCATCGCTTGGTCTATGCGATCGAAGATGGCGCGATACAGATCGTTATGTGC
AGATACCATTACTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT204598 WP_001307405.1 NZ_CP076043:c4005810-4005475 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT204598 NZ_CP101125:c179438-179184 [Pseudomonas nunensis]
ATGCAAACCATCAACTACACCACTGCGCGTGCACACTTGGCTGAAACCATGGATCGGGTCAATGAAGATCGTGCGCCCTT
GCTGGTAACTCGCCAGAAGGGCGAACCGGTGGTGATGATGTCACTAGCAGAGTACAACGCGCTCGAAGAGACCGCATATT
TGCTCCGATCCCCAGCCAATGCTGAACGCTTGATCAAATCAATTGGCAACCTGCGAGCAGGGAAAACCAAGGCCAGGCGC
CTTATCGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References