Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2433074..2433615 Replicon chromosome
Accession NZ_CP076043
Organism Escherichia coli strain Stbl4

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag ITN70_RS11940 Protein ID WP_000615983.1
Coordinates 2433337..2433615 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag ITN70_RS11935 Protein ID WP_000729703.1
Coordinates 2433074..2433334 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ITN70_RS21950 (2428605) 2428605..2428774 - 170 Protein_2334 hypothetical protein -
ITN70_RS11915 (2429019) 2429019..2430032 - 1014 WP_001013499.1 4-hydroxy-2-oxovalerate aldolase -
ITN70_RS11920 (2430022) 2430022..2431218 + 1197 Protein_2336 FHIPEP family type III secretion protein -
ITN70_RS11925 (2431442) 2431442..2431939 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
ITN70_RS11930 (2432115) 2432115..2432864 - 750 WP_000056849.1 C40 family peptidase -
ITN70_RS11935 (2433074) 2433074..2433334 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
ITN70_RS11940 (2433337) 2433337..2433615 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
ITN70_RS11945 (2433771) 2433771..2434511 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
ITN70_RS11950 (2434482) 2434482..2435249 - 768 WP_000333380.1 class II glutamine amidotransferase -
ITN70_RS11955 (2435455) 2435455..2436033 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T204594 WP_000615983.1 NZ_CP076043:2433337-2433615 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T204594 NZ_CP101124:c2011565-2011203 [Staphylococcus aureus subsp. aureus RN4220]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT204594 WP_000729703.1 NZ_CP076043:2433074-2433334 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT204594 NZ_CP101124:c2011732-2011562 [Staphylococcus aureus subsp. aureus RN4220]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References