Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1118963..1119613 Replicon chromosome
Accession NZ_CP075881
Organism Klebsiella michiganensis strain JNQH491

Toxin (Protein)


Gene name higB Uniprot ID A0A0H3H3K0
Locus tag KLH67_RS05155 Protein ID WP_014227314.1
Coordinates 1118963..1119304 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H3H3Q5
Locus tag KLH67_RS05160 Protein ID WP_014227313.1
Coordinates 1119314..1119613 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KLH67_RS05140 (KLH67_05140) 1115226..1116545 + 1320 WP_014227316.1 MFS transporter -
KLH67_RS05145 (KLH67_05145) 1116691..1118082 + 1392 WP_004126843.1 hexose-6-phosphate:phosphate antiporter -
KLH67_RS05150 (KLH67_05150) 1118260..1118712 + 453 WP_009653860.1 DUF1198 domain-containing protein -
KLH67_RS05155 (KLH67_05155) 1118963..1119304 + 342 WP_014227314.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KLH67_RS05160 (KLH67_05160) 1119314..1119613 + 300 WP_014227313.1 helix-turn-helix transcriptional regulator Antitoxin
KLH67_RS05165 (KLH67_05165) 1119732..1120913 + 1182 WP_049084188.1 purine ribonucleoside efflux pump NepI -
KLH67_RS05170 (KLH67_05170) 1120946..1121755 - 810 WP_049084182.1 phosphonoacetaldehyde hydrolase -
KLH67_RS05175 (KLH67_05175) 1121765..1122868 - 1104 WP_048262986.1 2-aminoethylphosphonate--pyruvate transaminase -
KLH67_RS05180 (KLH67_05180) 1123009..1123728 + 720 WP_009653851.1 phosphonate utilization transcriptional regulator PhnR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13110.00 Da        Isoelectric Point: 5.7545

>T204439 WP_014227314.1 NZ_CP075881:1118963-1119304 [Klebsiella michiganensis]
MWDVETTEVFDKWFEAQTEALKEDMLAAMVILSEYGPRLGRPFADTVNDSAFSNMKELRIQHQGRPIRAFFVFDPSRCGI
VLCAGDKTGLNEKRFYKDMIKLADAEYRKHLNQ

Download         Length: 342 bp

>T204439 NZ_CP100728:887410-887808 [Salmonella enterica subsp. enterica serovar Blockley]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACGTCAAAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCCGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAGTTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10966.87 Da        Isoelectric Point: 9.7256

>AT204439 WP_014227313.1 NZ_CP075881:1119314-1119613 [Klebsiella michiganensis]
MATLKELMAKQSPESQARIAEKVEEMRQAIALSMLREELNMSQAELAKAMGVKQPTIAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPTGKRIAFHL

Download         Length: 300 bp

>AT204439 NZ_CP100728:887183-887410 [Salmonella enterica subsp. enterica serovar Blockley]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGATGATGTGAA
ACATGTCGAAATCATTGCGGTTGGCAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H3K0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3Q5

References