Detailed information of TA system TA204431

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 82716..83141
• Replicon: plasmid pS19_651-a
• Accession: NZ_CP075853
• Organism: Escherichia coli strain S19-651

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JFY28_RS25645
• Protein ID: WP_223195199.1
• Coordinates: 82716..82838 (-)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	82930..83141 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFY28_RS25610 (77790)	77790..78017	-	228	WP_001442099.1	conjugal transfer relaxosome protein TraY	-
JFY28_RS25615 (78111)	78111..78797	-	687	WP_000332490.1	PAS domain-containing protein	-
JFY28_RS25620 (78987)	78987..79370	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
JFY28_RS25625 (79692)	79692..80294	+	603	Protein_95	transglycosylase SLT domain-containing protein	-
JFY28_RS25630 (80590)	80590..81411	-	822	WP_001234468.1	DUF932 domain-containing protein	-
JFY28_RS25635 (81530)	81530..81817	-	288	WP_000107520.1	hypothetical protein	-
JFY28_RS25640 (82118)	82118..82415	+	298	Protein_98	hypothetical protein	-
JFY28_RS25645 (82716)	82716..82838	-	123	WP_223195199.1	Hok/Gef family protein	Toxin
JFY28_RS26830 (82783)	82783..82896	-	114	Protein_100	DUF5431 family protein	-
- (82930)	82930..83141	-	212	NuclAT_0	-	Antitoxin
- (82930)	82930..83141	-	212	NuclAT_0	-	Antitoxin
- (82930)	82930..83141	-	212	NuclAT_0	-	Antitoxin
- (82930)	82930..83141	-	212	NuclAT_0	-	Antitoxin
JFY28_RS25650 (83086)	83086..83872	-	787	Protein_101	plasmid SOS inhibition protein A	-
JFY28_RS25655 (83869)	83869..84303	-	435	WP_000845922.1	conjugation system SOS inhibitor PsiB	-
JFY28_RS25660 (84358)	84358..86316	-	1959	WP_025670724.1	ParB/RepB/Spo0J family partition protein	-
JFY28_RS25665 (86380)	86380..86613	-	234	WP_001553739.1	DUF905 family protein	-
JFY28_RS25670 (86671)	86671..87210	-	540	WP_025670725.1	single-stranded DNA-binding protein	-
JFY28_RS25675 (87236)	87236..87442	-	207	WP_000547969.1	hypothetical protein	-
JFY28_RS25680 (87512)	87512..87937	+	426	WP_071791129.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3'')-Ib / aph(6)-Id / sitABCD	iutA / iucD / iucC / iucB / iucA	1..117341	117341

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4536.35 Da
• Isoelectric Point: 8.2691

>T204431 WP_223195199.1 NZ_CP075853:c82838-82716 [Escherichia coli]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 123 bp

>T204431 NZ_CP100724:4562522-4562833 [Salmonella enterica subsp. enterica serovar Enteritidis]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACACTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGCACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATGATCGGGGTAATAAAGAATGA

Antitoxin

Nucleotide

• Length: 212 bp

>AT204431 NZ_CP075853:c83141-82930 [Escherichia coli]
TCACACGGATTACCCGTGAACTGGTTGAACGACCAGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Antitoxin