Detailed information of TA system TA204428

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 44644..44898
• Replicon: plasmid pS19_651-a
• Accession: NZ_CP075853
• Organism: Escherichia coli strain S19-651

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: JFY28_RS25425
• Protein ID: WP_001312851.1
• Coordinates: 44644..44793 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	44837..44898 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFY28_RS25385 (40162)	40162..40341	+	180	Protein_45	IS91 family transposase	-
JFY28_RS25390 (40527)	40527..41048	+	522	Protein_46	IS3-like element IS600 family transposase	-
JFY28_RS25395 (41092)	41092..41301	-	210	Protein_47	type II toxin-antitoxin system RelE/ParE family toxin	-
JFY28_RS25400 (41301)	41301..41585	-	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	-
JFY28_RS25405 (42496)	42496..43329	-	834	WP_175109978.1	incFII family plasmid replication initiator RepA	-
JFY28_RS25410 (43322)	43322..43804	-	483	WP_001273588.1	hypothetical protein	-
JFY28_RS26815 (43797)	43797..43844	-	48	WP_229471593.1	hypothetical protein	-
JFY28_RS26820 (43835)	43835..44086	+	252	WP_223195197.1	replication protein RepA	-
JFY28_RS25420 (44103)	44103..44360	-	258	WP_000083845.1	replication regulatory protein RepA	-
JFY28_RS25425 (44644)	44644..44793	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (44837)	44837..44898	+	62	NuclAT_1	-	Antitoxin
- (44837)	44837..44898	+	62	NuclAT_1	-	Antitoxin
- (44837)	44837..44898	+	62	NuclAT_1	-	Antitoxin
- (44837)	44837..44898	+	62	NuclAT_1	-	Antitoxin
JFY28_RS25430 (45154)	45154..45228	-	75	Protein_55	endonuclease	-
JFY28_RS25435 (45474)	45474..45686	-	213	WP_001299730.1	ANR family transcriptional regulator	-
JFY28_RS25440 (45822)	45822..46382	-	561	WP_025670714.1	fertility inhibition protein FinO	-
JFY28_RS25445 (46485)	46485..47345	-	861	WP_000704513.1	alpha/beta hydrolase	-
JFY28_RS25450 (47404)	47404..48150	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3'')-Ib / aph(6)-Id / sitABCD	iutA / iucD / iucC / iucB / iucA	1..117341	117341

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T204428 WP_001312851.1 NZ_CP075853:c44793-44644 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T204428 NZ_CP100724:c4112665-4112381 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGCAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT204428 NZ_CP075853:44837-44898 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin