Detailed information of TA system TA204414

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3361911..3362710
• Replicon: chromosome
• Accession: NZ_CP075852
• Organism: Escherichia coli strain S19-651

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: U9XVR9
• Locus tag: JFY28_RS16375
• Protein ID: WP_000347267.1
• Coordinates: 3361911..3362375 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: JFY28_RS16380
• Protein ID: WP_032331352.1
• Coordinates: 3362375..3362710 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFY28_RS16345 (3356912)	3356912..3357346	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
JFY28_RS16350 (3357364)	3357364..3358242	-	879	WP_001298758.1	PTS N-acetylgalactosamine transporter subunit IID	-
JFY28_RS16355 (3358232)	3358232..3359011	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
JFY28_RS16360 (3359022)	3359022..3359495	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
JFY28_RS16365 (3359518)	3359518..3360798	-	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
JFY28_RS16370 (3361047)	3361047..3361856	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
JFY28_RS16375 (3361911)	3361911..3362375	-	465	WP_000347267.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
JFY28_RS16380 (3362375)	3362375..3362710	-	336	WP_032331352.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
JFY28_RS16385 (3362859)	3362859..3364430	-	1572	WP_001273741.1	galactarate dehydratase	-
JFY28_RS16390 (3364805)	3364805..3366139	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
JFY28_RS16395 (3366155)	3366155..3366925	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17807.17 Da
• Isoelectric Point: 9.4947

>T204414 WP_000347267.1 NZ_CP075852:c3362375-3361911 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T204414 NZ_CP100720:8673-8960 [Salmonella enterica subsp. enterica serovar Weltevreden]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12334.96 Da
• Isoelectric Point: 4.6672

>AT204414 WP_032331352.1 NZ_CP075852:c3362710-3362375 [Escherichia coli]
MPANARSLAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT204414 NZ_CP100720:8434-8673 [Salmonella enterica subsp. enterica serovar Weltevreden]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAAGCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0XTR4