Detailed information of TA system TA204406

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 1640578..1641119
• Replicon: chromosome
• Accession: NZ_CP075852
• Organism: Escherichia coli strain S19-651

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: U9XPG0
• Locus tag: JFY28_RS08260
• Protein ID: WP_000615976.1
• Coordinates: 1640841..1641119 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: JFY28_RS08255
• Protein ID: WP_032331203.1
• Coordinates: 1640578..1640838 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFY28_RS08230 (1636361)	1636361..1637146	-	786	WP_000207589.1	putative lateral flagellar export/assembly protein LafU	-
JFY28_RS08235 (1637118)	1637118..1638830	+	1713	Protein_1622	flagellar biosynthesis protein FlhA	-
JFY28_RS08240 (1638935)	1638935..1639213	+	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
JFY28_RS08245 (1639206)	1639206..1639562	+	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-
JFY28_RS08250 (1639619)	1639619..1640392	-	774	WP_000543896.1	C40 family peptidase	-
JFY28_RS08255 (1640578)	1640578..1640838	+	261	WP_032331203.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
JFY28_RS08260 (1640841)	1640841..1641119	+	279	WP_000615976.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
JFY28_RS08265 (1641275)	1641275..1642015	+	741	WP_001225679.1	peptidoglycan meso-diaminopimelic acid protein amidase	-
JFY28_RS08270 (1641986)	1641986..1642753	-	768	WP_000333380.1	class II glutamine amidotransferase	-
JFY28_RS08275 (1642959)	1642959..1643537	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-84)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10800.59 Da
• Isoelectric Point: 10.0702

>T204406 WP_000615976.1 NZ_CP075852:1640841-1641119 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T204406 NZ_CP100718:c4282885-4282577 [Salmonella enterica subsp. enterica serovar Weltevreden]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGATTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGACGGGTTCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9421.80 Da
• Isoelectric Point: 5.0758

>AT204406 WP_032331203.1 NZ_CP075852:1640578-1640838 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSETGVDVHKAKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT204406 NZ_CP100718:c4283127-4282888 [Salmonella enterica subsp. enterica serovar Weltevreden]
ATGTCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTGGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCAGCGGTGCTTACCGCCGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Structures

Toxin

• AlphaFold DB: U9XPG0