Detailed information of TA system TA204399

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 29278..29547
• Replicon: plasmid pCPF6-IncFII
• Accession: NZ_CP075741
• Organism: Escherichia coli strain CPF6

Toxin (Protein)

• Gene name: hok
• Uniprot ID: G9G195
• Locus tag: KKZ13_RS25170
• Protein ID: WP_001323520.1
• Coordinates: 29431..29547 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	29278..29343 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKZ13_RS25130	24312..24572	+	261	WP_071600428.1	hypothetical protein	-
KKZ13_RS25135	25045..25584	+	540	WP_000290791.1	single-stranded DNA-binding protein	-
KKZ13_RS25140	25647..25880	+	234	WP_000005990.1	DUF905 family protein	-
KKZ13_RS25145	25946..27904	+	1959	WP_023148339.1	ParB/RepB/Spo0J family partition protein	-
KKZ13_RS25150	27959..28393	+	435	WP_000845934.1	conjugation system SOS inhibitor PsiB	-
KKZ13_RS25155	28390..29152	+	763	Protein_40	plasmid SOS inhibition protein A	-
KKZ13_RS25160	29121..29309	-	189	WP_001299721.1	hypothetical protein	-
-	29121..29345	+	225	NuclAT_0	-	-
-	29121..29345	+	225	NuclAT_0	-	-
-	29121..29345	+	225	NuclAT_0	-	-
-	29121..29345	+	225	NuclAT_0	-	-
-	29278..29343	-	66	-	-	Antitoxin
KKZ13_RS25165	29331..29480	+	150	Protein_42	plasmid maintenance protein Mok	-
KKZ13_RS25170	29431..29547	+	117	WP_001323520.1	type I toxin-antitoxin system Hok family toxin	Toxin
KKZ13_RS25175	29767..29997	+	231	WP_001426396.1	hypothetical protein	-
KKZ13_RS25180	29995..30168	-	174	Protein_45	hypothetical protein	-
KKZ13_RS25185	30238..30444	+	207	WP_000547968.1	hypothetical protein	-
KKZ13_RS25190	30469..30756	+	288	WP_065800506.1	hypothetical protein	-
KKZ13_RS25195	30874..31695	+	822	WP_001234469.1	DUF932 domain-containing protein	-
KKZ13_RS25200	31992..32582	-	591	WP_165899088.1	transglycosylase SLT domain-containing protein	-
KKZ13_RS25205	32915..33298	+	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KKZ13_RS25210	33485..34174	+	690	WP_000283380.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4455.23 Da
• Isoelectric Point: 8.5110

>T204399 WP_001323520.1 NZ_CP075741:29431-29547 [Escherichia coli]
VCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 117 bp

>T204399 NZ_CP100718:883217-883630 [Salmonella enterica subsp. enterica serovar Weltevreden]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGTCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT204399 NZ_CP075741:c29343-29278 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Toxin

• AlphaFold DB: G9G195

Antitoxin