Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4007168..4007709 Replicon chromosome
Accession NZ_CP075737
Organism Escherichia coli strain CPF6

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag KKZ13_RS19120 Protein ID WP_000615983.1
Coordinates 4007168..4007446 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag KKZ13_RS19125 Protein ID WP_000729703.1
Coordinates 4007449..4007709 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKZ13_RS19105 (4004750) 4004750..4005328 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
KKZ13_RS19110 (4005534) 4005534..4006301 + 768 WP_000333380.1 class II glutamine amidotransferase -
KKZ13_RS19115 (4006272) 4006272..4007012 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KKZ13_RS19120 (4007168) 4007168..4007446 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KKZ13_RS19125 (4007449) 4007449..4007709 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KKZ13_RS19130 (4007919) 4007919..4008668 + 750 WP_000056849.1 C40 family peptidase -
KKZ13_RS19135 (4008844) 4008844..4009341 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
KKZ13_RS19140 (4009464) 4009464..4011176 - 1713 Protein_3727 flagellar biosynthesis protein FlhA -
KKZ13_RS19145 (4011148) 4011148..4011933 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T204389 WP_000615983.1 NZ_CP075737:c4007446-4007168 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T204389 NZ_CP100715:3473331-3473549 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT204389 WP_000729703.1 NZ_CP075737:c4007709-4007449 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT204389 NZ_CP100715:3472929-3473303 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References