Detailed information of TA system TA204364

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 71448..71874
• Replicon: plasmid pCP52E-IncFIB
• Accession: NZ_CP075732
• Organism: Escherichia coli strain CP52E

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KKZ11_RS23530
• Protein ID: WP_001372321.1
• Coordinates: 71448..71573 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	71650..71874 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKZ11_RS23490 (66601)	66601..67002	-	402	WP_001369361.1	conjugal transfer relaxosome DNA-bindin protein TraY	-
KKZ11_RS23495 (67095)	67095..67784	-	690	WP_039002187.1	conjugal transfer transcriptional regulator TraJ	-
KKZ11_RS23500 (67971)	67971..68354	-	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KKZ11_RS23505 (68675)	68675..69277	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
KKZ11_RS23510 (69574)	69574..70395	-	822	WP_058682586.1	DUF932 domain-containing protein	-
KKZ11_RS23515 (70514)	70514..70801	-	288	WP_000107535.1	hypothetical protein	-
KKZ11_RS23520 (70803)	70803..71000	+	198	Protein_67	hypothetical protein	-
KKZ11_RS23525 (70998)	70998..71228	-	231	WP_001426396.1	hypothetical protein	-
KKZ11_RS23530 (71448)	71448..71573	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KKZ11_RS23535 (71515)	71515..71664	-	150	Protein_70	plasmid maintenance protein Mok	-
- (71650)	71650..71874	-	225	NuclAT_0	-	Antitoxin
- (71650)	71650..71874	-	225	NuclAT_0	-	Antitoxin
- (71650)	71650..71874	-	225	NuclAT_0	-	Antitoxin
- (71650)	71650..71874	-	225	NuclAT_0	-	Antitoxin
KKZ11_RS23540 (71686)	71686..71874	+	189	WP_001299721.1	hypothetical protein	-
KKZ11_RS23545 (71843)	71843..72605	-	763	Protein_72	plasmid SOS inhibition protein A	-
KKZ11_RS23550 (72602)	72602..73036	-	435	WP_256631400.1	conjugation system SOS inhibitor PsiB	-
KKZ11_RS23555 (73091)	73091..75049	-	1959	WP_021528456.1	ParB/RepB/Spo0J family partition protein	-
KKZ11_RS23560 (75115)	75115..75348	-	234	WP_000005990.1	DUF905 family protein	-
KKZ11_RS23565 (75411)	75411..75950	-	540	WP_000290839.1	single-stranded DNA-binding protein	-
KKZ11_RS23570 (75976)	75976..76182	-	207	WP_000275853.1	hypothetical protein	-
KKZ11_RS23575 (76423)	76423..76656	-	234	WP_071940851.1	hypothetical protein	-
KKZ11_RS23580 (76593)	76593..76781	-	189	WP_102360578.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / dfrA14	vat	1..115794	115794

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T204364 WP_001372321.1 NZ_CP075732:c71573-71448 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T204364 NZ_CP100709:c47982-47830 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT204364 NZ_CP075732:c71874-71650 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin