Detailed information of TA system TA204358

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4621317..4621919
• Replicon: chromosome
• Accession: NZ_CP075731
• Organism: Escherichia coli strain CP52E

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KKZ11_RS22270
• Protein ID: WP_000897305.1
• Coordinates: 4621608..4621919 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KKZ11_RS22265
• Protein ID: WP_000356397.1
• Coordinates: 4621317..4621607 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKZ11_RS22240 (4617245)	4617245..4618147	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
KKZ11_RS22245 (4618144)	4618144..4618779	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
KKZ11_RS22250 (4618776)	4618776..4619705	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
KKZ11_RS22255 (4620034)	4620034..4620276	-	243	WP_024199136.1	CopG family transcriptional regulator	-
KKZ11_RS22260 (4620495)	4620495..4620713	-	219	WP_001295676.1	CopG family transcriptional regulator	-
KKZ11_RS22265 (4621317)	4621317..4621607	-	291	WP_000356397.1	NadS family protein	Antitoxin
KKZ11_RS22270 (4621608)	4621608..4621919	-	312	WP_000897305.1	hypothetical protein	Toxin
KKZ11_RS22275 (4622148)	4622148..4623056	+	909	WP_136698623.1	alpha/beta hydrolase	-
KKZ11_RS22280 (4623225)	4623225..4624139	-	915	WP_122992729.1	transposase	-
KKZ11_RS22285 (4624152)	4624152..4625039	-	888	Protein_4355	hypothetical protein	-
KKZ11_RS22290 (4625454)	4625454..4626395	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KKZ11_RS22295 (4626440)	4626440..4626877	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T204358 WP_000897305.1 NZ_CP075731:c4621919-4621608 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T204358 NZ_CP100707:c4194944-4194660 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT204358 WP_000356397.1 NZ_CP075731:c4621607-4621317 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT204358 NZ_CP100707:c4195176-4194934 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7