Detailed information of TA system TA204339

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1847155..1847657
• Replicon: chromosome
• Accession: NZ_CP075731
• Organism: Escherichia coli strain CP52E

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: KKZ11_RS08750
• Protein ID: WP_000767835.1
• Coordinates: 1847403..1847657 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: KKZ11_RS08745
• Protein ID: WP_001259255.1
• Coordinates: 1847155..1847406 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKZ11_RS08720 (1842332)	1842332..1843399	-	1068	WP_000080072.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
KKZ11_RS08725 (1843399)	1843399..1844469	-	1071	WP_000108941.1	histidinol-phosphate transaminase	-
KKZ11_RS08730 (1844466)	1844466..1845770	-	1305	WP_001413482.1	histidinol dehydrogenase	-
KKZ11_RS08735 (1845776)	1845776..1846675	-	900	WP_000131774.1	ATP phosphoribosyltransferase	-
KKZ11_RS08740 (1846821)	1846821..1846871	-	51	WP_001364200.1	his operon leader peptide	-
KKZ11_RS08745 (1847155)	1847155..1847406	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
KKZ11_RS08750 (1847403)	1847403..1847657	+	255	WP_000767835.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
KKZ11_RS08755 (1847740)	1847740..1848564	+	825	WP_000754737.1	SDR family oxidoreductase	-
KKZ11_RS08760 (1848610)	1848610..1849539	+	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
KKZ11_RS08765 (1849754)	1849754..1849816	+	63	WP_010723108.1	membrane protein YoeI	-
KKZ11_RS08770 (1849806)	1849806..1851164	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
KKZ11_RS08775 (1851343)	1851343..1852401	+	1059	WP_000492321.1	thiosulfate utilization transporter TsuA/YeeE	-
KKZ11_RS08780 (1852415)	1852415..1852642	+	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10169.51 Da
• Isoelectric Point: 7.2751

>T204339 WP_000767835.1 NZ_CP075731:1847403-1847657 [Escherichia coli]
VKLIWSEESWDDYLYWQETDNRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T204339 NZ_CP100702:3413402-3413620 [Salmonella enterica subsp. enterica serovar Thompson]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTATCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT204339 WP_001259255.1 NZ_CP075731:1847155-1847406 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT204339 NZ_CP100702:3413000-3413374 [Salmonella enterica subsp. enterica serovar Thompson]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0