Detailed information of TA system TA204334

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 39220..39489
• Replicon: plasmid pE35-IncFII
• Accession: NZ_CP075725
• Organism: Escherichia coli strain CPE35

Toxin (Protein)

• Gene name: hok
• Uniprot ID: G9G195
• Locus tag: KKZ10_RS23830
• Protein ID: WP_001323520.1
• Coordinates: 39373..39489 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	39220..39285 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKZ10_RS23790	34248..34514	+	267	WP_071828223.1	hypothetical protein	-
KKZ10_RS23795	34987..35526	+	540	WP_000290791.1	single-stranded DNA-binding protein	-
KKZ10_RS23800	35589..35822	+	234	WP_000005990.1	DUF905 family protein	-
KKZ10_RS23805	35888..37846	+	1959	WP_023148339.1	ParB/RepB/Spo0J family partition protein	-
KKZ10_RS23810	37901..38335	+	435	WP_000845934.1	conjugation system SOS inhibitor PsiB	-
KKZ10_RS23815	38332..39094	+	763	Protein_54	plasmid SOS inhibition protein A	-
KKZ10_RS23820	39063..39251	-	189	WP_001299721.1	hypothetical protein	-
-	39063..39287	+	225	NuclAT_0	-	-
-	39063..39287	+	225	NuclAT_0	-	-
-	39063..39287	+	225	NuclAT_0	-	-
-	39063..39287	+	225	NuclAT_0	-	-
-	39220..39285	-	66	-	-	Antitoxin
KKZ10_RS23825	39273..39422	+	150	Protein_56	plasmid maintenance protein Mok	-
KKZ10_RS23830	39373..39489	+	117	WP_001323520.1	type I toxin-antitoxin system Hok family toxin	Toxin
KKZ10_RS23835	39709..39939	+	231	WP_001426396.1	hypothetical protein	-
KKZ10_RS23840	39937..40110	-	174	Protein_59	hypothetical protein	-
KKZ10_RS23845	40180..40386	+	207	WP_000547968.1	hypothetical protein	-
KKZ10_RS23850	40411..40698	+	288	WP_065800506.1	hypothetical protein	-
KKZ10_RS23855	40816..41637	+	822	WP_001234469.1	DUF932 domain-containing protein	-
KKZ10_RS23860	41934..42524	-	591	WP_165899088.1	transglycosylase SLT domain-containing protein	-
KKZ10_RS23865	42857..43240	+	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KKZ10_RS23870	43427..44116	+	690	WP_000283380.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4455.23 Da
• Isoelectric Point: 8.5110

>T204334 WP_001323520.1 NZ_CP075725:39373-39489 [Escherichia coli]
VCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 117 bp

>T204334 NZ_CP100702:834778-835191 [Salmonella enterica subsp. enterica serovar Thompson]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT204334 NZ_CP075725:c39285-39220 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Toxin

• AlphaFold DB: G9G195

Antitoxin