Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3611386..3611927 Replicon chromosome
Accession NZ_CP075722
Organism Escherichia coli strain CPE35

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag KKZ10_RS17560 Protein ID WP_000615983.1
Coordinates 3611649..3611927 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag KKZ10_RS17555 Protein ID WP_000729703.1
Coordinates 3611386..3611646 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKZ10_RS17535 (3607061) 3607061..3607846 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
KKZ10_RS17540 (3607818) 3607818..3609530 + 1713 Protein_3428 flagellar biosynthesis protein FlhA -
KKZ10_RS17545 (3609754) 3609754..3610251 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
KKZ10_RS17550 (3610427) 3610427..3611176 - 750 WP_000056849.1 C40 family peptidase -
KKZ10_RS17555 (3611386) 3611386..3611646 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KKZ10_RS17560 (3611649) 3611649..3611927 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KKZ10_RS17565 (3612083) 3612083..3612823 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KKZ10_RS17570 (3612794) 3612794..3613561 - 768 WP_000333380.1 class II glutamine amidotransferase -
KKZ10_RS17575 (3613767) 3613767..3614345 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T204329 WP_000615983.1 NZ_CP075722:3611649-3611927 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T204329 NZ_CP100698:4605867-4606178 [Salmonella enterica subsp. enterica serovar Agona]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTAACTGCATATTATCGGGGTAATAAAGAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT204329 WP_000729703.1 NZ_CP075722:3611386-3611646 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT204329 NZ_CP100698:4606175-4606621 [Salmonella enterica subsp. enterica serovar Agona]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGCTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTCAAACTACCAGCAGATGCCTTCATCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References