Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3605189..3605883 Replicon chromosome
Accession NZ_CP075722
Organism Escherichia coli strain CPE35

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag KKZ10_RS17520 Protein ID WP_001263489.1
Coordinates 3605189..3605587 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag KKZ10_RS17525 Protein ID WP_000554758.1
Coordinates 3605590..3605883 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3600777) 3600777..3600857 - 81 NuclAT_11 - -
- (3600777) 3600777..3600857 - 81 NuclAT_11 - -
- (3600777) 3600777..3600857 - 81 NuclAT_11 - -
- (3600777) 3600777..3600857 - 81 NuclAT_11 - -
KKZ10_RS17495 (3601453) 3601453..3601911 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
KKZ10_RS17500 (3602172) 3602172..3603629 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
KKZ10_RS17505 (3603686) 3603686..3604207 - 522 Protein_3421 peptide chain release factor H -
KKZ10_RS17510 (3604203) 3604203..3604409 - 207 Protein_3422 RtcB family protein -
KKZ10_RS17515 (3604727) 3604727..3605179 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
KKZ10_RS17520 (3605189) 3605189..3605587 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
KKZ10_RS17525 (3605590) 3605590..3605883 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
KKZ10_RS17530 (3605935) 3605935..3606990 - 1056 WP_001226164.1 DNA polymerase IV -
KKZ10_RS17535 (3607061) 3607061..3607846 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
KKZ10_RS17540 (3607818) 3607818..3609530 + 1713 Protein_3428 flagellar biosynthesis protein FlhA -
KKZ10_RS17545 (3609754) 3609754..3610251 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T204328 WP_001263489.1 NZ_CP075722:c3605587-3605189 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T204328 NZ_CP100698:c4604634-4604323 [Salmonella enterica subsp. enterica serovar Agona]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGATCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT204328 WP_000554758.1 NZ_CP075722:c3605883-3605590 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT204328 NZ_CP100698:c4604322-4604032 [Salmonella enterica subsp. enterica serovar Agona]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGGTTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGGTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References