Detailed information of TA system TA204305

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 35457..35726
• Replicon: plasmid pCPWW7-IncFII
• Accession: NZ_CP075718
• Organism: Escherichia coli strain CPww7

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KKZ15_RS24840
• Protein ID: WP_001372321.1
• Coordinates: 35601..35726 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	35457..35522 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKZ15_RS24805	31167..31694	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
KKZ15_RS24810	31752..31985	+	234	WP_000006003.1	DUF905 family protein	-
KKZ15_RS24815	32046..34069	+	2024	Protein_43	ParB/RepB/Spo0J family partition protein	-
KKZ15_RS24820	34138..34572	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
KKZ15_RS24825	34569..35331	+	763	Protein_45	plasmid SOS inhibition protein A	-
-	35300..35524	+	225	NuclAT_0	-	-
-	35300..35524	+	225	NuclAT_0	-	-
-	35300..35524	+	225	NuclAT_0	-	-
-	35300..35524	+	225	NuclAT_0	-	-
KKZ15_RS24830	35309..35488	-	180	WP_001309233.1	hypothetical protein	-
-	35457..35522	-	66	-	-	Antitoxin
KKZ15_RS24835	35510..35659	+	150	Protein_47	plasmid maintenance protein Mok	-
KKZ15_RS24840	35601..35726	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KKZ15_RS24845	36028..37599	-	1572	WP_094083869.1	IS66-like element ISCro1 family transposase	-
KKZ15_RS24850	37619..37966	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
KKZ15_RS24855	37966..38616	-	651	WP_000993956.1	IS66-like element accessory protein TnpA	-
KKZ15_RS24860	38757..39053	-	297	Protein_52	hypothetical protein	-
KKZ15_RS24865	39353..39649	+	297	WP_001272251.1	hypothetical protein	-
KKZ15_RS24870	39760..40581	+	822	WP_001234445.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T204305 WP_001372321.1 NZ_CP075718:35601-35726 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T204305 NZ_CP100695:3582442-3582660 [Salmonella enterica subsp. enterica serovar Weltevreden]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT204305 NZ_CP075718:c35522-35457 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin