Detailed information of TA system TA204301

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 133259..133684
• Replicon: plasmid pCPWW7-IncF
• Accession: NZ_CP075717
• Organism: Escherichia coli strain CPww7

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KKZ15_RS24435
• Protein ID: WP_223195199.1
• Coordinates: 133259..133381 (-)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	133473..133684 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKZ15_RS24400 (128421)	128421..129242	-	822	WP_061856956.1	DUF932 domain-containing protein	-
KKZ15_RS24405 (129361)	129361..129648	-	288	WP_000107543.1	hypothetical protein	-
KKZ15_RS24410 (129673)	129673..129879	-	207	WP_024189527.1	hypothetical protein	-
KKZ15_RS24415 (129949)	129949..130246	+	298	Protein_140	hypothetical protein	-
KKZ15_RS24420 (130344)	130344..130994	+	651	WP_000993956.1	IS66-like element accessory protein TnpA	-
KKZ15_RS24425 (130994)	130994..131341	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
KKZ15_RS24430 (131361)	131361..132932	+	1572	WP_094083869.1	IS66-like element ISCro1 family transposase	-
KKZ15_RS24435 (133259)	133259..133381	-	123	WP_223195199.1	Hok/Gef family protein	Toxin
KKZ15_RS24440 (133326)	133326..133439	-	114	Protein_145	DUF5431 family protein	-
- (133473)	133473..133684	-	212	NuclAT_0	-	Antitoxin
- (133473)	133473..133684	-	212	NuclAT_0	-	Antitoxin
- (133473)	133473..133684	-	212	NuclAT_0	-	Antitoxin
- (133473)	133473..133684	-	212	NuclAT_0	-	Antitoxin
KKZ15_RS24445 (133629)	133629..134415	-	787	Protein_146	plasmid SOS inhibition protein A	-
KKZ15_RS24450 (134412)	134412..134846	-	435	WP_000845926.1	conjugation system SOS inhibitor PsiB	-
KKZ15_RS24455 (134901)	134901..136860	-	1960	Protein_148	ParB/RepB/Spo0J family partition protein	-
KKZ15_RS24460 (136926)	136926..137159	-	234	WP_000006002.1	DUF905 family protein	-
KKZ15_RS24465 (137217)	137217..137645	-	429	WP_063115590.1	single-stranded DNA-binding protein	-
KKZ15_RS24470 (138345)	138345..138536	-	192	WP_025269849.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..162438	162438

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4536.35 Da
• Isoelectric Point: 8.2691

>T204301 WP_223195199.1 NZ_CP075717:c133381-133259 [Escherichia coli]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 123 bp

>T204301 NZ_CP100695:848262-848675 [Salmonella enterica subsp. enterica serovar Weltevreden]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGTCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin

Nucleotide

• Length: 212 bp

>AT204301 NZ_CP075717:c133684-133473 [Escherichia coli]
TCACACGGATTACCCGTGAACTGGTTGAACGACCAGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Antitoxin