Detailed information of TA system TA204298

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 88575..88829
• Replicon: plasmid pCPWW7-IncF
• Accession: NZ_CP075717
• Organism: Escherichia coli strain CPww7

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: KKZ15_RS24170
• Protein ID: WP_074152653.1
• Coordinates: 88575..88724 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	88768..88829 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKZ15_RS24125 (83625)	83625..84776	-	1152	WP_195686664.1	IS30-like element IS30 family transposase	-
KKZ15_RS24130 (84844)	84844..85146	-	303	Protein_83	transposase	-
KKZ15_RS24135 (85182)	85182..85379	-	198	Protein_84	3'-5' exonuclease	-
KKZ15_RS24140 (85419)	85419..85697	-	279	WP_001384452.1	type II toxin-antitoxin system toxin YacB	-
KKZ15_RS24145 (85697)	85697..85966	-	270	WP_000079923.1	type II toxin-antitoxin system antitoxin YacA	-
KKZ15_RS24150 (86873)	86873..87729	-	857	Protein_87	incFII family plasmid replication initiator RepA	-
KKZ15_RS24155 (87722)	87722..87796	-	75	WP_001365705.1	RepA leader peptide Tap	-
KKZ15_RS24160 (87845)	87845..87970	+	126	WP_001436741.1	hypothetical protein	-
KKZ15_RS24165 (88043)	88043..88291	-	249	WP_000083838.1	replication regulatory protein RepA	-
KKZ15_RS24170 (88575)	88575..88724	-	150	WP_074152653.1	Hok/Gef family protein	Toxin
- (88768)	88768..88829	+	62	NuclAT_1	-	Antitoxin
- (88768)	88768..88829	+	62	NuclAT_1	-	Antitoxin
- (88768)	88768..88829	+	62	NuclAT_1	-	Antitoxin
- (88768)	88768..88829	+	62	NuclAT_1	-	Antitoxin
KKZ15_RS24175 (89088)	89088..89387	-	300	WP_050593763.1	hypothetical protein	-
KKZ15_RS24180 (89505)	89505..89951	-	447	WP_063115548.1	thermonuclease family protein	-
KKZ15_RS24185 (90089)	90089..91702	-	1614	WP_047063389.1	IS66-like element ISEc23 family transposase	-
KKZ15_RS24190 (91733)	91733..92083	-	351	WP_001565133.1	IS66 family insertion sequence element accessory protein TnpB	-
KKZ15_RS24195 (92080)	92080..92505	-	426	WP_000422741.1	transposase	-
KKZ15_RS24200 (93234)	93234..93437	-	204	WP_001327131.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..162438	162438
	-	inside	IScluster/Tn	-	-	81337..92505	11168

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5558.67 Da
• Isoelectric Point: 8.7678

>T204298 WP_074152653.1 NZ_CP075717:c88724-88575 [Escherichia coli]
MTKYTLIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T204298 NZ_CP100693:c4530584-4530273 [Salmonella enterica subsp. enterica serovar London]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT204298 NZ_CP075717:88768-88829 [Escherichia coli]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin