Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3705904..3706703 Replicon chromosome
Accession NZ_CP075716
Organism Escherichia coli strain CPww7

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag KKZ15_RS18125 Protein ID WP_000347267.1
Coordinates 3705904..3706368 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KKZ15_RS18130 Protein ID WP_001307405.1
Coordinates 3706368..3706703 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKZ15_RS18095 (3700905) 3700905..3701339 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KKZ15_RS18100 (3701357) 3701357..3702235 - 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
KKZ15_RS18105 (3702225) 3702225..3703004 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KKZ15_RS18110 (3703015) 3703015..3703488 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
KKZ15_RS18115 (3703511) 3703511..3704791 - 1281 WP_000681947.1 tagatose-bisphosphate aldolase subunit KbaZ -
KKZ15_RS18120 (3705040) 3705040..3705849 + 810 WP_000072180.1 aga operon transcriptional regulator AgaR -
KKZ15_RS18125 (3705904) 3705904..3706368 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KKZ15_RS18130 (3706368) 3706368..3706703 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KKZ15_RS18135 (3706852) 3706852..3708423 - 1572 WP_001273741.1 galactarate dehydratase -
KKZ15_RS18140 (3708798) 3708798..3710132 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KKZ15_RS18145 (3710148) 3710148..3710918 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T204293 WP_000347267.1 NZ_CP075716:c3706368-3705904 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T204293 NZ_CP100693:3411164-3411382 [Salmonella enterica subsp. enterica serovar London]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT204293 WP_001307405.1 NZ_CP075716:c3706703-3706368 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT204293 NZ_CP100693:3410762-3411136 [Salmonella enterica subsp. enterica serovar London]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References