204289

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2602097..2602594 Replicon chromosome
Accession NZ_CP075716
Organism Escherichia coli strain CPww7

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KKZ15_RS12795 Protein ID WP_001173343.1
Coordinates 2602097..2602270 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KKZ15_RS12800 Protein ID WP_000398619.1
Coordinates 2602298..2602594 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKZ15_RS12785 (2598767) 2598767..2600224 + 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -
KKZ15_RS12790 (2600461) 2600461..2601978 + 1518 WP_001295087.1 lysine--tRNA ligase -
KKZ15_RS12795 (2602097) 2602097..2602270 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KKZ15_RS12800 (2602298) 2602298..2602594 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KKZ15_RS12805 (2602821) 2602821..2603093 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
KKZ15_RS12810 (2603105) 2603105..2603335 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
KKZ15_RS12815 (2603516) 2603516..2605147 + 1632 WP_001216487.1 sensor histidine kinase -
KKZ15_RS12820 (2605144) 2605144..2605863 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2593243..2602594 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T204289 WP_001173343.1 NZ_CP075716:c2602270-2602097 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T204289 NZ_CP100693:843482-843895 [Salmonella enterica subsp. enterica serovar London]
GTGATCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT204289 WP_000398619.1 NZ_CP075716:c2602594-2602298 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT204289 NZ_CP100693:843235-843501 [Salmonella enterica subsp. enterica serovar London]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGATCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References