Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2420..3066 Replicon plasmid pSTEC2018_607-f
Accession NZ_CP075703
Organism Escherichia coli strain STEC2018-607

Toxin (Protein)


Gene name higB Uniprot ID F4T8Q4
Locus tag JFX52_RS27660 Protein ID WP_000269926.1
Coordinates 2716..3066 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID F4T8Q5
Locus tag JFX52_RS27655 Protein ID WP_001259443.1
Coordinates 2420..2719 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX52_RS27630 (JFX52_27630) 1..287 + 287 WP_252356246.1 hypothetical protein -
JFX52_RS27635 (JFX52_27635) 658..1191 + 534 WP_001025389.1 J domain-containing protein -
JFX52_RS27640 (JFX52_27640) 1319..1861 + 543 WP_000206647.1 hypothetical protein -
JFX52_RS27645 (JFX52_27645) 1897..2154 + 258 WP_000845367.1 hypothetical protein -
JFX52_RS27650 (JFX52_27650) 2144..2389 + 246 WP_001193619.1 hypothetical protein -
JFX52_RS27655 (JFX52_27655) 2420..2719 - 300 WP_001259443.1 XRE family transcriptional regulator Antitoxin
JFX52_RS27660 (JFX52_27660) 2716..3066 - 351 WP_000269926.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JFX52_RS27665 (JFX52_27665) 3432..4079 + 648 WP_000214917.1 ParA family protein -
JFX52_RS27670 (JFX52_27670) 4119..4352 + 234 WP_000024515.1 hypothetical protein -
JFX52_RS27675 (JFX52_27675) 4384..4647 - 264 WP_001312455.1 PilI type IV pilus biogenesis protein -
JFX52_RS28490 4826..5131 - 306 Protein_10 hypothetical protein -
JFX52_RS27685 (JFX52_27685) 5141..5605 - 465 WP_000697615.1 thermonuclease family protein -
JFX52_RS27690 (JFX52_27690) 5609..6343 - 735 WP_000905147.1 MobC family replication-relaxation protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..24412 24412


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-80)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13742.77 Da        Isoelectric Point: 7.4217

>T204274 WP_000269926.1 NZ_CP075703:c3066-2716 [Escherichia coli]
MWTVYFGRLFDEWFEEQELALKRKVLAELKHLEEFGPSLSRPHADTVKGSRHKNMKELRIQYEGHPIRAFFAFDPIRQAI
ILCAGDKSNDKKFYDRMIRIADEEFSTHLAELEGKK

Download         Length: 351 bp

>T204274 NZ_CP100690:83875-85200 [Salmonella enterica subsp. enterica serovar Newport]
ATGAGCCGGAAACAGCAACGTCTGGTAATTTGGATGAACGGCATCAAAGTCGGGTACTGGGAAAAAAGCAAAGGGGTAGA
CAGCTTAGAATACCTTCCTGAATGGGTTGCTGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTGCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTGCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACTGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGAGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGGCCAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAGACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCGTTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAGGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAATTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCAAACTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10917.70 Da        Isoelectric Point: 4.6283

>AT204274 WP_001259443.1 NZ_CP075703:c2719-2420 [Escherichia coli]
MRTLEEVIAAQSPESQAIIKAMADEMILETGLQMMREELQLSQKQVADAMGISQPAVTQLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRVAFNI

Download         Length: 300 bp

>AT204274 NZ_CP100690:83577-83870 [Salmonella enterica subsp. enterica serovar Newport]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGCTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGCGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCCATCCTGGGCGTAAAAATCAGCTTCTCCTCGGCAACCACCTCTTCAGAGAGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGGATTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A743U745

Antitoxin

Source ID Structure
AlphaFold DB A0A743U677

References