Detailed information of TA system TA204270

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 52174..52600
• Replicon: plasmid pSTEC2018_607-c
• Accession: NZ_CP075700
• Organism: Escherichia coli strain STEC2018-607

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JFX52_RS26860
• Protein ID: WP_001372321.1
• Coordinates: 52174..52299 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	52376..52600 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX52_RS26825 (47228)	47228..47443	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
JFX52_RS26830 (47579)	47579..48226	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
JFX52_RS26835 (48417)	48417..48800	-	384	WP_001063020.1	conjugal transfer relaxosome DNA-binding protein TraM	-
JFX52_RS26840 (49152)	49152..49742	+	591	WP_277996786.1	transglycosylase SLT domain-containing protein	-
JFX52_RS26845 (50038)	50038..50859	-	822	Protein_57	DUF932 domain-containing protein	-
JFX52_RS26850 (50979)	50979..51266	-	288	WP_000107542.1	hypothetical protein	-
JFX52_RS26855 (51291)	51291..51497	-	207	WP_032192990.1	hypothetical protein	-
JFX52_RS28440 (51567)	51567..51704	+	138	Protein_60	hypothetical protein	-
JFX52_RS28445 (51724)	51724..51954	-	231	WP_071998556.1	hypothetical protein	-
JFX52_RS26860 (52174)	52174..52299	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
JFX52_RS28450 (52241)	52241..52390	-	150	Protein_63	plasmid maintenance protein Mok	-
- (52376)	52376..52600	-	225	NuclAT_0	-	Antitoxin
- (52376)	52376..52600	-	225	NuclAT_0	-	Antitoxin
- (52376)	52376..52600	-	225	NuclAT_0	-	Antitoxin
- (52376)	52376..52600	-	225	NuclAT_0	-	Antitoxin
JFX52_RS26865 (52412)	52412..52600	+	189	WP_001299721.1	hypothetical protein	-
JFX52_RS26870 (52569)	52569..53331	-	763	Protein_65	plasmid SOS inhibition protein A	-
JFX52_RS26875 (53328)	53328..53762	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
JFX52_RS26880 (53817)	53817..55775	-	1959	WP_062859180.1	ParB/RepB/Spo0J family partition protein	-
JFX52_RS26885 (55841)	55841..56074	-	234	WP_000005990.1	DUF905 family protein	-
JFX52_RS26890 (56137)	56137..56676	-	540	WP_000290838.1	single-stranded DNA-binding protein	-
JFX52_RS26895 (56702)	56702..56908	-	207	WP_000275853.1	hypothetical protein	-
JFX52_RS28455 (57149)	57149..57421	-	273	WP_072118979.1	hypothetical protein	-
JFX52_RS28460 (57319)	57319..57555	-	237	WP_062876510.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iutA / iucD / iucC / iucB / iucA	1..87927	87927

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T204270 WP_001372321.1 NZ_CP075700:c52299-52174 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T204270 NZ_CP100689:c4119845-4119561 [Salmonella enterica subsp. enterica serovar Newport]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT204270 NZ_CP075700:c52600-52376 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin