Detailed information of TA system TA204266

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 13244..13498
• Replicon: plasmid pSTEC2018_607-c
• Accession: NZ_CP075700
• Organism: Escherichia coli strain STEC2018-607

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: JFX52_RS26640
• Protein ID: WP_001312851.1
• Coordinates: 13244..13393 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	13437..13498 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX52_RS26605 (8626)	8626..9854	+	1229	WP_198777257.1	IS3-like element IS2 family transposase	-
JFX52_RS26610 (10003)	10003..10290	-	288	WP_000865085.1	helix-turn-helix transcriptional regulator	-
JFX52_RS26615 (10290)	10290..10601	-	312	WP_000483535.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JFX52_RS26620 (10707)	10707..10847	-	141	WP_001315588.1	hypothetical protein	-
JFX52_RS26625 (11546)	11546..12403	-	858	WP_198777258.1	incFII family plasmid replication initiator RepA	-
JFX52_RS26630 (12396)	12396..12470	-	75	WP_001365705.1	RepA leader peptide Tap	-
JFX52_RS28435 (12496)	12496..12639	+	144	WP_071988605.1	replication protein RepA	-
JFX52_RS26635 (12712)	12712..12960	-	249	WP_000083838.1	replication regulatory protein RepA	-
JFX52_RS26640 (13244)	13244..13393	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (13437)	13437..13498	+	62	NuclAT_1	-	Antitoxin
- (13437)	13437..13498	+	62	NuclAT_1	-	Antitoxin
- (13437)	13437..13498	+	62	NuclAT_1	-	Antitoxin
- (13437)	13437..13498	+	62	NuclAT_1	-	Antitoxin
JFX52_RS26645 (13673)	13673..14056	-	384	WP_001109067.1	hypothetical protein	-
JFX52_RS26650 (14159)	14159..14620	-	462	WP_000760085.1	thermonuclease family protein	-
JFX52_RS26655 (15363)	15363..15566	-	204	WP_001327131.1	hypothetical protein	-
JFX52_RS26660 (15721)	15721..16278	-	558	WP_000840474.1	fertility inhibition protein FinO	-
JFX52_RS26665 (16381)	16381..17241	-	861	WP_000704525.1	alpha/beta hydrolase	-
JFX52_RS26670 (17300)	17300..18046	-	747	WP_000205769.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iutA / iucD / iucC / iucB / iucA	1..87927	87927

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T204266 WP_001312851.1 NZ_CP075700:c13393-13244 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T204266 NZ_CP100689:c1013538-1013011 [Salmonella enterica subsp. enterica serovar Newport]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGAAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT204266 NZ_CP075700:13437-13498 [Escherichia coli]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin