Detailed information of TA system TA204262

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 98696..98965
• Replicon: plasmid pSTEC2018_607-a
• Accession: NZ_CP075698
• Organism: Escherichia coli strain STEC2018-607

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JFX52_RS25975
• Protein ID: WP_001372321.1
• Coordinates: 98840..98965 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	98696..98761 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX52_RS28395	93798..94079	+	282	WP_071829438.1	hypothetical protein	-
JFX52_RS28400	94320..94526	+	207	WP_052078239.1	hypothetical protein	-
JFX52_RS25945	94552..95004	+	453	WP_000290842.1	single-stranded DNA-binding protein	-
JFX52_RS25950	95066..95299	+	234	WP_001519997.1	DUF905 family protein	-
JFX52_RS25955	95364..97322	+	1959	WP_063072993.1	ParB/RepB/Spo0J family partition protein	-
JFX52_RS25960	97377..97811	+	435	WP_000845907.1	conjugation system SOS inhibitor PsiB	-
JFX52_RS25965	97808..98570	+	763	Protein_101	plasmid SOS inhibition protein A	-
JFX52_RS25970	98539..98727	-	189	WP_001299721.1	hypothetical protein	-
-	98539..98763	+	225	NuclAT_0	-	-
-	98539..98763	+	225	NuclAT_0	-	-
-	98539..98763	+	225	NuclAT_0	-	-
-	98539..98763	+	225	NuclAT_0	-	-
-	98696..98761	-	66	-	-	Antitoxin
JFX52_RS28405	98749..98898	+	150	Protein_103	plasmid maintenance protein Mok	-
JFX52_RS25975	98840..98965	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
JFX52_RS28410	99185..99415	+	231	WP_071845785.1	hypothetical protein	-
JFX52_RS28415	99413..99586	-	174	Protein_106	hypothetical protein	-
JFX52_RS25980	99656..99862	+	207	WP_033804333.1	hypothetical protein	-
JFX52_RS25985	99887..100174	+	288	WP_000107542.1	hypothetical protein	-
JFX52_RS25990	100293..101114	+	822	WP_033804334.1	DUF932 domain-containing protein	-
JFX52_RS25995	101411..101722	-	312	Protein_110	transglycosylase SLT domain-containing protein	-
JFX52_RS26000	101778..102475	+	698	WP_223429336.1	IS1 family transposase	-
JFX52_RS26005	102491..102829	+	339	Protein_112	MFS transporter	-
JFX52_RS26010	102845..103666	+	822	WP_198777264.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	hlyC / hlyA / hlyB / hlyD / espP	1..106835	106835
	-	flank	IS/Tn	-	-	101972..102475	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T204262 WP_001372321.1 NZ_CP075698:98840-98965 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T204262 NZ_CP100687:7051-7305 [Salmonella enterica subsp. enterica serovar Goldcoast]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT204262 NZ_CP075698:c98761-98696 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin