Detailed information of TA system TA204245

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2455544..2456146
• Replicon: chromosome
• Accession: NZ_CP075697
• Organism: Escherichia coli strain STEC2018-607

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JFX52_RS11870
• Protein ID: WP_000897305.1
• Coordinates: 2455835..2456146 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JFX52_RS11865
• Protein ID: WP_000356397.1
• Coordinates: 2455544..2455834 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX52_RS11840 (2451488)	2451488..2452390	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JFX52_RS11845 (2452387)	2452387..2453022	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JFX52_RS11850 (2453019)	2453019..2453948	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
JFX52_RS11855 (2454278)	2454278..2454520	-	243	WP_001087409.1	protein YiiF	-
JFX52_RS11860 (2454740)	2454740..2454958	-	219	WP_001315930.1	CopG family transcriptional regulator	-
JFX52_RS11865 (2455544)	2455544..2455834	-	291	WP_000356397.1	NadS family protein	Antitoxin
JFX52_RS11870 (2455835)	2455835..2456146	-	312	WP_000897305.1	hypothetical protein	Toxin
JFX52_RS11875 (2456375)	2456375..2457283	+	909	WP_001162704.1	alpha/beta hydrolase	-
JFX52_RS11880 (2457347)	2457347..2458288	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JFX52_RS11885 (2458333)	2458333..2458770	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
JFX52_RS11890 (2458767)	2458767..2459639	-	873	WP_000920762.1	virulence factor BrkB family protein	-
JFX52_RS11895 (2459633)	2459633..2460232	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T204245 WP_000897305.1 NZ_CP075697:c2456146-2455835 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T204245 NZ_CP100682:3305566-3305784 [Salmonella enterica subsp. enterica serovar Goldcoast]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT204245 WP_000356397.1 NZ_CP075697:c2455834-2455544 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT204245 NZ_CP100682:3305164-3305538 [Salmonella enterica subsp. enterica serovar Goldcoast]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7