Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1613430..1613971 Replicon chromosome
Accession NZ_CP075697
Organism Escherichia coli strain STEC2018-607

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag JFX52_RS07990 Protein ID WP_000615976.1
Coordinates 1613693..1613971 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag JFX52_RS07985 Protein ID WP_000729704.1
Coordinates 1613430..1613690 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX52_RS07960 (1609213) 1609213..1609998 - 786 WP_000207589.1 putative lateral flagellar export/assembly protein LafU -
JFX52_RS07965 (1609970) 1609970..1611682 + 1713 Protein_1581 flagellar biosynthesis protein FlhA -
JFX52_RS07970 (1611787) 1611787..1612065 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
JFX52_RS07975 (1612058) 1612058..1612414 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
JFX52_RS07980 (1612471) 1612471..1613244 - 774 WP_000543896.1 C40 family peptidase -
JFX52_RS07985 (1613430) 1613430..1613690 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JFX52_RS07990 (1613693) 1613693..1613971 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JFX52_RS07995 (1614127) 1614127..1614867 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
JFX52_RS08000 (1614838) 1614838..1615605 - 768 WP_000333380.1 class II glutamine amidotransferase -
JFX52_RS08005 (1615811) 1615811..1616389 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T204240 WP_000615976.1 NZ_CP075697:1613693-1613971 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T204240 NZ_CP100682:213667-214152 [Salmonella enterica subsp. enterica serovar Goldcoast]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGGGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT204240 WP_000729704.1 NZ_CP075697:1613430-1613690 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT204240 NZ_CP100682:213392-213679 [Salmonella enterica subsp. enterica serovar Goldcoast]
ATGCTATACAAAGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCGGGCTGCCGAGAATGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATTAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References