Detailed information of TA system TA204211

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2284..2538
• Replicon: plasmid pSTEC2018_553-c
• Accession: NZ_CP075668
• Organism: Escherichia coli strain STEC2018-553

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: JFY30_RS28170
• Protein ID: WP_001312851.1
• Coordinates: 2284..2433 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	2482..2538 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFY30_RS28155 (592)	592..1449	-	858	WP_198789103.1	incFII family plasmid replication initiator RepA	-
JFY30_RS28160 (1442)	1442..1516	-	75	WP_001365705.1	RepA leader peptide Tap	-
JFY30_RS28165 (1740)	1740..2000	-	261	WP_000083819.1	replication regulatory protein RepA	-
JFY30_RS28170 (2284)	2284..2433	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (2482)	2482..2538	+	57	NuclAT_0	-	Antitoxin
- (2482)	2482..2538	+	57	NuclAT_0	-	Antitoxin
- (2482)	2482..2538	+	57	NuclAT_0	-	Antitoxin
- (2482)	2482..2538	+	57	NuclAT_0	-	Antitoxin
JFY30_RS28175 (2713)	2713..3111	-	399	WP_000675594.1	hypothetical protein	-
JFY30_RS28180 (3199)	3199..3660	-	462	WP_000760081.1	thermonuclease family protein	-
JFY30_RS28185 (4402)	4402..4605	-	204	WP_001336517.1	hypothetical protein	-
JFY30_RS28190 (4759)	4759..5316	-	558	WP_000139316.1	fertility inhibition protein FinO	-
JFY30_RS28195 (5419)	5419..6279	-	861	WP_000704512.1	alpha/beta hydrolase	-
JFY30_RS28200 (6338)	6338..7084	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	senB	1..36145	36145

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T204211 WP_001312851.1 NZ_CP075668:c2433-2284 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T204211 NZ_CP100670:c967430-967203 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGACCACCTGTTACTCACAAATCCCCAGCCAGCACCTTAAGGGTGACTGGCAGGAAGAAGCGGGATTTGAGACCGGGCA
CGGCGTCACCGTGAAGATCTCCGAGGGGTGTTTGATCCTGATTGCAGAGACGGACGAAGTGAGGGATCTACGGAAGGAGC
TTTATCAGGTGAAAAAGTCGATGAAGCATATTAAGGCGGGGGTAAATAATGTGGTGAACGGGGATTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT204211 NZ_CP075668:2482-2538 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin