Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4950107..4950648 Replicon chromosome
Accession NZ_CP075665
Organism Escherichia coli strain STEC2018-553

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag JFY30_RS24570 Protein ID WP_000615976.1
Coordinates 4950107..4950385 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag JFY30_RS24575 Protein ID WP_000729704.1
Coordinates 4950388..4950648 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFY30_RS24555 (4947689) 4947689..4948267 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
JFY30_RS24560 (4948473) 4948473..4949240 + 768 WP_000333380.1 class II glutamine amidotransferase -
JFY30_RS24565 (4949211) 4949211..4949951 - 741 WP_001225685.1 peptidoglycan meso-diaminopimelic acid protein amidase -
JFY30_RS24570 (4950107) 4950107..4950385 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JFY30_RS24575 (4950388) 4950388..4950648 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JFY30_RS24580 (4950834) 4950834..4951607 + 774 WP_000543896.1 C40 family peptidase -
JFY30_RS24585 (4951664) 4951664..4952020 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
JFY30_RS24590 (4952013) 4952013..4952291 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
JFY30_RS24595 (4952396) 4952396..4954108 - 1713 Protein_4840 flagellar biosynthesis protein FlhA -
JFY30_RS24600 (4954080) 4954080..4954865 + 786 WP_000207589.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T204203 WP_000615976.1 NZ_CP075665:c4950385-4950107 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T204203 NZ_CP100666:4744617-4744928 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT204203 WP_000729704.1 NZ_CP075665:c4950648-4950388 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT204203 NZ_CP100666:4744929-4745219 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References