Detailed information of TA system TA204199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3893348..3893950
• Replicon: chromosome
• Accession: NZ_CP075665
• Organism: Escherichia coli strain STEC2018-553

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JFY30_RS19410
• Protein ID: WP_000897305.1
• Coordinates: 3893348..3893659 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JFY30_RS19415
• Protein ID: WP_000356397.1
• Coordinates: 3893660..3893950 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFY30_RS19385 (3889262)	3889262..3889861	+	600	WP_001295269.1	glucose-1-phosphatase	-
JFY30_RS19390 (3889855)	3889855..3890727	+	873	WP_000920762.1	virulence factor BrkB family protein	-
JFY30_RS19395 (3890724)	3890724..3891161	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
JFY30_RS19400 (3891206)	3891206..3892147	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JFY30_RS19405 (3892211)	3892211..3893119	-	909	WP_198779597.1	alpha/beta hydrolase	-
JFY30_RS19410 (3893348)	3893348..3893659	+	312	WP_000897305.1	hypothetical protein	Toxin
JFY30_RS19415 (3893660)	3893660..3893950	+	291	WP_000356397.1	NadS family protein	Antitoxin
JFY30_RS19420 (3894536)	3894536..3894754	+	219	WP_001315930.1	CopG family transcriptional regulator	-
JFY30_RS19425 (3894974)	3894974..3895216	+	243	WP_001087409.1	protein YiiF	-
JFY30_RS19430 (3895546)	3895546..3896475	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
JFY30_RS19435 (3896472)	3896472..3897107	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JFY30_RS19440 (3897104)	3897104..3898006	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T204199 WP_000897305.1 NZ_CP075665:3893348-3893659 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T204199 NZ_CP100666:c4194927-4194652 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT204199 WP_000356397.1 NZ_CP075665:3893660-3893950 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT204199 NZ_CP100666:c4195190-4194930 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCACGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7