Detailed information of TA system TA204176

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 41297..41551
• Replicon: plasmid pSTEC17_197-a
• Accession: NZ_CP075664
• Organism: Escherichia coli strain STEC2017-197

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A1D7QA06
• Locus tag: JFX55_RS24570
• Protein ID: WP_001367749.1
• Coordinates: 41297..41446 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	41490..41551 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX55_RS24540 (36920)	36920..37342	-	423	WP_001212724.1	Exc2 family lipoprotein	-
JFX55_RS24545 (37625)	37625..38052	+	428	Protein_38	transposase	-
JFX55_RS24550 (38383)	38383..38622	-	240	WP_000859018.1	winged helix-turn-helix transcriptional regulator	-
JFX55_RS24555 (39595)	39595..40452	-	858	WP_000130938.1	incFII family plasmid replication initiator RepA	-
JFX55_RS24560 (40445)	40445..40519	-	75	WP_001367777.1	RepA leader peptide Tap	-
JFX55_RS24565 (40765)	40765..41013	-	249	WP_000083842.1	replication regulatory protein RepA	-
JFX55_RS24570 (41297)	41297..41446	-	150	WP_001367749.1	Hok/Gef family protein	Toxin
- (41490)	41490..41551	+	62	NuclAT_0	-	Antitoxin
- (41490)	41490..41551	+	62	NuclAT_0	-	Antitoxin
- (41490)	41490..41551	+	62	NuclAT_0	-	Antitoxin
- (41490)	41490..41551	+	62	NuclAT_0	-	Antitoxin
JFX55_RS24575 (41726)	41726..42109	-	384	WP_001109066.1	hypothetical protein	-
JFX55_RS24580 (42212)	42212..42673	-	462	WP_000417388.1	thermonuclease family protein	-
JFX55_RS24585 (43418)	43418..43621	-	204	WP_001327131.1	hypothetical protein	-
JFX55_RS24590 (43775)	43775..44338	-	564	WP_000139382.1	fertility inhibition protein FinO	-
JFX55_RS24595 (44441)	44441..45301	-	861	WP_000868985.1	alpha/beta hydrolase	-
JFX55_RS24600 (45360)	45360..46100	-	741	Protein_49	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC	1..118523	118523

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5528.64 Da
• Isoelectric Point: 8.7678

>T204176 WP_001367749.1 NZ_CP075664:c41446-41297 [Escherichia coli]
MTKYALIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T204176 NZ_CP100653:1918320-1918907 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGGCGTTGCCGCTCGCCGTCCGCCTCGGTGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAGTTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGCGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGCATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGCGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGCGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT204176 NZ_CP075664:41490-41551 [Escherichia coli]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A1D7QA06

Antitoxin