Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3816730..3817529 Replicon chromosome
Accession NZ_CP075663
Organism Escherichia coli strain STEC2017-197

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag JFX55_RS18710 Protein ID WP_000347267.1
Coordinates 3816730..3817194 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JFX55_RS18715 Protein ID WP_001307405.1
Coordinates 3817194..3817529 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX55_RS18680 (3811731) 3811731..3812165 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
JFX55_RS18685 (3812183) 3812183..3813061 - 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
JFX55_RS18690 (3813051) 3813051..3813830 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
JFX55_RS18695 (3813841) 3813841..3814314 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JFX55_RS18700 (3814337) 3814337..3815617 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
JFX55_RS18705 (3815866) 3815866..3816675 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JFX55_RS18710 (3816730) 3816730..3817194 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JFX55_RS18715 (3817194) 3817194..3817529 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JFX55_RS18720 (3817678) 3817678..3819249 - 1572 WP_001273741.1 galactarate dehydratase -
JFX55_RS18725 (3819624) 3819624..3820958 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
JFX55_RS18730 (3820974) 3820974..3821744 + 771 WP_001058232.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T204171 WP_000347267.1 NZ_CP075663:c3817194-3816730 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T204171 NZ_CP100652:c5273310-5272996 [Pseudomonas putida]
ATGATCTTCATAGAAACGCCCATTTTTACCCAAGATGTGAAGGAACACTTGGGTGACGATGAGTATCGGGAACTGCAGTC
GTACCTCGCTGGGCAGCCAATCGCTGGAGACCTTATAGAGGGTACGGGTGGCCTAAGGAAAATCCGCTGGTCGGCCAAGG
GCAAAGGCAAGAGTGGTGGCGTGCGAGTGATTTATTACCATGTATCCTCGGCATACCAGATACGAATGATTCTGATCTAT
CGCAAGGGCATCATGGATACCTTGACTGATAAACAGAAAGCGCAGCTTCGCGCAATCAACAAAGGGTGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT204171 WP_001307405.1 NZ_CP075663:c3817529-3817194 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT204171 NZ_CP100652:c5272996-5272709 [Pseudomonas putida]
ATGGAAAAGAATCTCTTCGATCGTCTAGTGGAAAGCATGACACAGATGGATGAGATCGATCGCGGTGAGCGTCAGCCTTC
GCGCGAGTTCCACGTTGACGCGGTTCAGGTCAAAAAAATCCGCCAAGCAACTGGTCTCTCCCAGGCTGCGTTCGCCAAAC
GCATCGATGTTGCTGTAGGGACCCTACGAAATTGGGAACAAGGCCGCCGCGAGCCGGAAGGCCCCGCTCGTGCTCTGCTT
CGTGCAATACACAATGACCCTGAGCATGTGCTTGCAGCATTGAGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References