Detailed information of TA system TA204169

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3021661..3022263
• Replicon: chromosome
• Accession: NZ_CP075663
• Organism: Escherichia coli strain STEC2017-197

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JFX55_RS14860
• Protein ID: WP_000897305.1
• Coordinates: 3021952..3022263 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JFX55_RS14855
• Protein ID: WP_000356397.1
• Coordinates: 3021661..3021951 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX55_RS14830 (3017226)	3017226..3017861	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JFX55_RS14835 (3017858)	3017858..3018787	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
JFX55_RS14840 (3019187)	3019187..3020167	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
JFX55_RS14845 (3020396)	3020396..3020638	-	243	WP_001087409.1	protein YiiF	-
JFX55_RS14850 (3020857)	3020857..3021075	-	219	WP_001295676.1	CopG family transcriptional regulator	-
JFX55_RS14855 (3021661)	3021661..3021951	-	291	WP_000356397.1	NadS family protein	Antitoxin
JFX55_RS14860 (3021952)	3021952..3022263	-	312	WP_000897305.1	hypothetical protein	Toxin
JFX55_RS14865 (3022492)	3022492..3023400	+	909	WP_001367979.1	alpha/beta hydrolase	-
JFX55_RS14870 (3023464)	3023464..3024405	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JFX55_RS14875 (3024450)	3024450..3024887	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
JFX55_RS14880 (3024884)	3024884..3025756	-	873	WP_000920762.1	virulence factor BrkB family protein	-
JFX55_RS14885 (3025750)	3025750..3026349	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3019187..3020167	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T204169 WP_000897305.1 NZ_CP075663:c3022263-3021952 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T204169 NZ_CP100652:c3809211-3808915 [Pseudomonas putida]
ATGGAAAAGAGAACGCCTCATTGTTCGCTGGAGCGGATCAGGGCCTTGGTCAGCGCTGGGCGCATCAGCCCGACAACCGC
CTCTTTGCGGGGCGCCCAGGCACTGGGGATGGATTACCCGGACATGCTCGAGATAATCAACGGTCTGGAGCGCAGAGACT
TTCAAAAAAGCATGACATGTCACGGTGATTACCGCGTATGGCAGGACGTTTATCGCCCCCTTACGGGCAAGGGATACGTG
TACCTGAAACTGTCTGTGGTGGATGACGTGCTCATCGTGTCTTTCAAGGAGTTGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT204169 WP_000356397.1 NZ_CP075663:c3021951-3021661 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT204169 NZ_CP100652:c3808912-3808511 [Pseudomonas putida]
ATGAAGTGTCCAGTTTGTGGGGGGGCGGAGCTTGCACCTGATACCCGGGACATGCCCTATCGATACAAAGGTGAAGTGAC
CGTTATCCCAAATGTCAGTGGCGGATACTGTACAGCCTGTGGGGAGGCGGTTCTCAGCCATGATGAAGCGATGCGCATCA
GTCCTTTGATGTCCGCATTCAATCGCCAAGTCAATGCCGCGGCAGTGGACCCCGACTTCATTGTCGCTGTGCGCAAGAAA
TTCGACCTCGACCAGCGTGAAGCCGGCGAAATCTTTGGCGGTGGGGTCAATGCGTTCTCTCGCTACGAAAATGGGAAAAC
CAGGCCGCCGGTTGCTCTGGTCAAACTGTTCAAGCTGCTCGACCGTCATCCGGAGCTGTTCGAGGAAGTCCGTACGGCCT
GA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7