Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2199645..2200186 Replicon chromosome
Accession NZ_CP075663
Organism Escherichia coli strain STEC2017-197

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag JFX55_RS11080 Protein ID WP_000615982.1
Coordinates 2199908..2200186 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag JFX55_RS11075 Protein ID WP_000729704.1
Coordinates 2199645..2199905 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX55_RS11050 (2195428) 2195428..2196213 - 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -
JFX55_RS11055 (2196185) 2196185..2197897 + 1713 Protein_2171 flagellar biosynthesis protein FlhA -
JFX55_RS11060 (2198002) 2198002..2198280 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
JFX55_RS11065 (2198273) 2198273..2198629 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
JFX55_RS11070 (2198686) 2198686..2199459 - 774 WP_000543899.1 C40 family peptidase -
JFX55_RS11075 (2199645) 2199645..2199905 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JFX55_RS11080 (2199908) 2199908..2200186 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JFX55_RS11085 (2200342) 2200342..2201082 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
JFX55_RS11090 (2201053) 2201053..2201820 - 768 WP_000333380.1 class II glutamine amidotransferase -
JFX55_RS11095 (2202026) 2202026..2202604 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T204167 WP_000615982.1 NZ_CP075663:2199908-2200186 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T204167 NZ_CP100651:c2270256-2270080 [Bacillus halotolerans]
GTGCTTGGGAAAGTGGGTATCATAGTTGCTTTCCTCATATCTTTAACGGTTCTTACAATCAACAGTCTAACAATAGTTGA
GAAGATAAGAAACCTAAAGAATGGGACAAGCAAAAAGAAAAAGCGTATACGCAAGCGGCTCCGACCAAAGAGACAACGCC
AACGTATACGCCGATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT204167 WP_000729704.1 NZ_CP075663:2199645-2199905 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT204167 NZ_CP100651:2270039-2270139 [Bacillus halotolerans]
TAGGAACTAAAGGAGAAGTTCATTCCCCTTTAGCTTAGCTCTCATCGGCGTATACGTTGGCGTTGTCTCTTTGGTCGGAG
CCGCTTGCGTATACGCTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References