Detailed information of TA system TA204146

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 87110..87522
• Replicon: plasmid pSTEC17_183-a
• Accession: NZ_CP075653
• Organism: Escherichia coli strain STEC17-183

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JFX43_RS25205
• Protein ID: WP_223200913.1
• Coordinates: 87110..87232 (-)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	87317..87522 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX43_RS25180 (82889)	82889..83272	-	384	WP_021521311.1	conjugal transfer relaxosome DNA-binding protein TraM	-
JFX43_RS25185 (83603)	83603..84205	+	603	WP_198800492.1	transglycosylase SLT domain-containing protein	-
JFX43_RS25190 (84501)	84501..85322	-	822	WP_198800504.1	DUF932 domain-containing protein	-
JFX43_RS25195 (85441)	85441..85728	-	288	WP_000107526.1	hypothetical protein	-
JFX43_RS25200 (85884)	85884..86186	-	303	WP_001399758.1	hypothetical protein	-
JFX43_RS26700 (86657)	86657..86887	-	231	WP_123009337.1	hypothetical protein	-
JFX43_RS25205 (87110)	87110..87232	-	123	WP_223200913.1	Hok/Gef family protein	Toxin
JFX43_RS26705 (87177)	87177..87329	-	153	Protein_104	DUF5431 family protein	-
- (87317)	87317..87522	-	206	NuclAT_0	-	Antitoxin
- (87317)	87317..87522	-	206	NuclAT_0	-	Antitoxin
- (87317)	87317..87522	-	206	NuclAT_0	-	Antitoxin
- (87317)	87317..87522	-	206	NuclAT_0	-	Antitoxin
JFX43_RS25210 (87479)	87479..87793	-	315	WP_001562885.1	hypothetical protein	-
JFX43_RS25215 (87790)	87790..88509	-	720	WP_001562886.1	plasmid SOS inhibition protein A	-
JFX43_RS25220 (88506)	88506..88940	-	435	WP_198800491.1	conjugation system SOS inhibitor PsiB	-
JFX43_RS25225 (89009)	89009..90957	-	1949	Protein_108	ParB/RepB/Spo0J family partition protein	-
JFX43_RS25230 (91022)	91022..91255	-	234	WP_000006021.1	DUF905 domain-containing protein	-
JFX43_RS25235 (91313)	91313..91834	-	522	WP_000290783.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyD / hlyB / hlyB / hlyA / hlyA / hlyA / hlyA / hlyC	1..111930	111930

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4538.38 Da
• Isoelectric Point: 8.2691

>T204146 WP_223200913.1 NZ_CP075653:c87232-87110 [Escherichia coli]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 123 bp

>T204146 NZ_CP100596:c2736474-2736133 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTAGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Nucleotide

• Length: 206 bp

>AT204146 NZ_CP075653:c87522-87317 [Escherichia coli]
CCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGTACGCAGGTATATGGGCTATGATGTG
CCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTATCCTGTCGTGTGGCAGAAGGACAAAAGCCCCGT
AGTTAATTTTTCATTAACCCACGAGGCCTCTGCATGTCTAGTCCAC

Structures

Antitoxin