Detailed information of TA system TA204129

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3345224..3346023
• Replicon: chromosome
• Accession: NZ_CP075652
• Organism: Escherichia coli strain STEC17-183

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: U9XVR9
• Locus tag: JFX43_RS16260
• Protein ID: WP_000347267.1
• Coordinates: 3345224..3345688 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: JFX43_RS16265
• Protein ID: WP_032331352.1
• Coordinates: 3345688..3346023 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX43_RS16230 (3340225)	3340225..3340659	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
JFX43_RS16235 (3340677)	3340677..3341555	-	879	WP_001298758.1	PTS N-acetylgalactosamine transporter subunit IID	-
JFX43_RS16240 (3341545)	3341545..3342324	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
JFX43_RS16245 (3342335)	3342335..3342808	-	474	WP_198800428.1	PTS N-acetylgalactosamine transporter subunit IIB	-
JFX43_RS16250 (3342831)	3342831..3344111	-	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
JFX43_RS16255 (3344360)	3344360..3345169	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
JFX43_RS16260 (3345224)	3345224..3345688	-	465	WP_000347267.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
JFX43_RS16265 (3345688)	3345688..3346023	-	336	WP_032331352.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
JFX43_RS16270 (3346172)	3346172..3347743	-	1572	WP_001273741.1	galactarate dehydratase	-
JFX43_RS16275 (3348118)	3348118..3349452	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
JFX43_RS16280 (3349468)	3349468..3350238	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17807.17 Da
• Isoelectric Point: 9.4947

>T204129 WP_000347267.1 NZ_CP075652:c3345688-3345224 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T204129 NZ_CP100544:c4198175-4198002 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12334.96 Da
• Isoelectric Point: 4.6672

>AT204129 WP_032331352.1 NZ_CP075652:c3346023-3345688 [Escherichia coli]
MPANARSLAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT204129 NZ_CP100544:c4198499-4198203 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A0E0XTR4