Detailed information of TA system TA204126

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2477494..2478096
• Replicon: chromosome
• Accession: NZ_CP075652
• Organism: Escherichia coli strain STEC17-183

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JFX43_RS12080
• Protein ID: WP_000897305.1
• Coordinates: 2477785..2478096 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JFX43_RS12075
• Protein ID: WP_000356397.1
• Coordinates: 2477494..2477784 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX43_RS12050 (2473438)	2473438..2474340	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JFX43_RS12055 (2474337)	2474337..2474972	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JFX43_RS12060 (2474969)	2474969..2475898	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
JFX43_RS12065 (2476228)	2476228..2476470	-	243	WP_001087409.1	protein YiiF	-
JFX43_RS12070 (2476690)	2476690..2476908	-	219	WP_001315930.1	CopG family transcriptional regulator	-
JFX43_RS12075 (2477494)	2477494..2477784	-	291	WP_000356397.1	NadS family protein	Antitoxin
JFX43_RS12080 (2477785)	2477785..2478096	-	312	WP_000897305.1	hypothetical protein	Toxin
JFX43_RS12085 (2478325)	2478325..2479233	+	909	WP_001162704.1	alpha/beta hydrolase	-
JFX43_RS12090 (2479297)	2479297..2480238	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JFX43_RS12095 (2480283)	2480283..2480720	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
JFX43_RS12100 (2480717)	2480717..2481589	-	873	WP_000920762.1	virulence factor BrkB family protein	-
JFX43_RS12105 (2481583)	2481583..2482183	-	601	Protein_2375	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T204126 WP_000897305.1 NZ_CP075652:c2478096-2477785 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T204126 NZ_CP100544:3655230-3655508 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT204126 WP_000356397.1 NZ_CP075652:c2477784-2477494 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT204126 NZ_CP100544:3654967-3655227 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7