Detailed information of TA system TA204115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4956825..4957427
• Replicon: chromosome
• Accession: NZ_CP075649
• Organism: Escherichia coli strain STEC17-190

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JFX56_RS23645
• Protein ID: WP_000897305.1
• Coordinates: 4957116..4957427 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JFX56_RS23640
• Protein ID: WP_000356397.1
• Coordinates: 4956825..4957115 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX56_RS23615 (4952751)	4952751..4953653	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JFX56_RS23620 (4953650)	4953650..4954285	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JFX56_RS23625 (4954282)	4954282..4955211	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
JFX56_RS23630 (4955541)	4955541..4955783	-	243	WP_001086388.1	protein YiiF	-
JFX56_RS23635 (4956002)	4956002..4956220	-	219	WP_001295676.1	CopG family transcriptional regulator	-
JFX56_RS23640 (4956825)	4956825..4957115	-	291	WP_000356397.1	NadS family protein	Antitoxin
JFX56_RS23645 (4957116)	4957116..4957427	-	312	WP_000897305.1	hypothetical protein	Toxin
JFX56_RS23650 (4957656)	4957656..4958564	+	909	WP_001162704.1	alpha/beta hydrolase	-
JFX56_RS23655 (4958628)	4958628..4959569	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JFX56_RS23660 (4959614)	4959614..4960051	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
JFX56_RS23665 (4960048)	4960048..4960920	-	873	WP_000920762.1	virulence factor BrkB family protein	-
JFX56_RS23670 (4960914)	4960914..4961513	-	600	WP_001315111.1	glucose-1-phosphatase	-
JFX56_RS23675 (4961612)	4961612..4962397	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T204115 WP_000897305.1 NZ_CP075649:c4957427-4957116 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T204115 NZ_CP100544:c589589-589125 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT204115 WP_000356397.1 NZ_CP075649:c4957115-4956825 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT204115 NZ_CP100544:c589924-589589 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7