Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4003028..4003569 Replicon chromosome
Accession NZ_CP075649
Organism Escherichia coli strain STEC17-190

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag JFX56_RS19230 Protein ID WP_000615976.1
Coordinates 4003291..4003569 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag JFX56_RS19225 Protein ID WP_000729704.1
Coordinates 4003028..4003288 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX56_RS19200 (3998811) 3998811..3999596 - 786 WP_000207590.1 putative lateral flagellar export/assembly protein LafU -
JFX56_RS19205 (3999568) 3999568..4001280 + 1713 Protein_3788 flagellar biosynthesis protein FlhA -
JFX56_RS19210 (4001385) 4001385..4001663 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
JFX56_RS19215 (4001656) 4001656..4002012 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
JFX56_RS19220 (4002069) 4002069..4002842 - 774 WP_136755766.1 C40 family peptidase -
JFX56_RS19225 (4003028) 4003028..4003288 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JFX56_RS19230 (4003291) 4003291..4003569 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JFX56_RS19235 (4003725) 4003725..4004465 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
JFX56_RS19240 (4004436) 4004436..4005203 - 768 WP_000333380.1 class II glutamine amidotransferase -
JFX56_RS19245 (4005409) 4005409..4005987 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 4001656..4013181 11525


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10782.56 Da        Isoelectric Point: 10.0702

>T204107 WP_000615976.1 NZ_CP075649:4003291-4003569 [Escherichia coli]
IIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T204107 NZ_CP100542:c4539152-4538619 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGAG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGTATTCTATCTTCGTCTGGGGTTTATACAGCTCGTAGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT204107 WP_000729704.1 NZ_CP075649:4003028-4003288 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT204107 NZ_CP100542:c4539429-4539163 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCTTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References