Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 649209..650008 Replicon chromosome
Accession NZ_CP075649
Organism Escherichia coli strain STEC17-190

Toxin (Protein)


Gene name yhaV Uniprot ID D3GWU5
Locus tag JFX56_RS03155 Protein ID WP_000347272.1
Coordinates 649209..649673 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JFX56_RS03160 Protein ID WP_001307405.1
Coordinates 649673..650008 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX56_RS03125 (644210) 644210..644644 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
JFX56_RS03130 (644662) 644662..645540 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
JFX56_RS03135 (645530) 645530..646309 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
JFX56_RS03140 (646320) 646320..646793 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JFX56_RS03145 (646816) 646816..648096 - 1281 WP_000681955.1 tagatose-bisphosphate aldolase subunit KbaZ -
JFX56_RS03150 (648345) 648345..649154 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
JFX56_RS03155 (649209) 649209..649673 - 465 WP_000347272.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JFX56_RS03160 (649673) 649673..650008 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JFX56_RS03165 (650157) 650157..651728 - 1572 WP_001273741.1 galactarate dehydratase -
JFX56_RS03170 (652103) 652103..653437 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
JFX56_RS03175 (653453) 653453..654223 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17832.26 Da        Isoelectric Point: 9.6924

>T204087 WP_000347272.1 NZ_CP075649:c649673-649209 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

>T204087 NZ_CP100530:2814515-2814622 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT204087 WP_001307405.1 NZ_CP075649:c650008-649673 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT204087 NZ_CP100530:c2814468-2814402 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829KUD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References